摘要
目的 应用彗星试验方法检测长期接触低浓度二硫化碳 (CS2 )职业人群的口腔黏膜细胞DNA损伤以及损伤程度。方法 在湖北省某大型化纤厂随机抽取 90例CS2 接触工人作为暴露组 ,同时选择 81例非接触工人作为对照组 ,进行彗星试验。结果 暴露组和对照组的彗星细胞拖尾率分别为 0 5 1%和 0 2 3% ,经 χ2 检验 ,差异有非常显著性。另外 ,低作业年限暴露组 (0 5 0 % )和男性暴露组 (0 5 6 % )的彗星细胞拖尾率均非常显著高于对照组 ,分别为 0 0 8%和 0 2 5 %。多因素非条件logistic回归分析结果表明 ,CS2 暴露组DNA损伤的可能性明显高于对照组。结论 长期接触低浓度CS2 对工人口腔黏膜细胞的DNA有一定损伤。
Objective DNA damage in human buccal cells of workers occupationally long-term exposed to carbon disulfide(CS_2)was monitored with comet assay. Methods Ninety workers exposed to CS_2 were randomly selected as exposure group from a large-scale chemical fiber manufacturer in Hubei and 81 workers not exposed to CS_2 as control group. DNA damage in their buccal cells was detected with comet assay. Results Rate of DNA tail was 0.51% in exposure group, significantly higher than that in control group (0.23%), with very statistical significance. Rate of DNA tail was 0.50% in male exposure group and 0.56% in exposure group with short length of employment, significantly higher than that in control group (0.08% and 0.25%, respectively). Multivariate unconditional logistic regression anlaysis showed that possibility of DNA damage was significantly higher in exposure group than that in control group ( P <0.05). Conclusion Long-term exposure to lower concentration of carbon disulfide could cause certain damage to human buccal cell DNA.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2004年第1期36-38,F004,共4页
Chinese Journal of Preventive Medicine
基金
教育部课题基金 (3 0 115 1968)
