摘要
目的 建立基因芯片快速检测人白细胞抗原A1 9(HLA A1 9)分型方法。方法 利用基因芯片技术 ,根据HLA A位点不同基因亚型的独特序列设计探针 ,制成分型芯片 ;待检测样品经聚合酶链反应 (PCR)标记上荧光之后 ,与芯片进行杂交 ,根据杂交产生的荧光信号值 ,分析确定样品A位点的基因亚型。对 1 2 0份移植供受者的HLA A基因分型 ,并对 1 1份样品作基因测序。结果 仅用 2 5h ,HLA A1 9基因分型芯片可准确分辨出A1 9抗原 9大类 (A2 90 1、A2 9XX、A30 0 1、A30 0 6、A30XX、A31、A32、A34和A74 )。结论 HLA A1 9组基因芯片分型方法 ,分辨率高、特异性强、重复性好、操作简便、结果直观 。
Objective To study Microarray technique for rapid genotyping HLA A Methods According to the sequence of HLA A exon2 and exon3, HLA A genotyping Chip was made and labeled PCR products hybridized with them The signals were sanned by sanner and analyzed by Imagene software We had genotyped 120 individuals of donor recipients and sequenced eleven samples Results The experimental results showed that the HLA A19 genotyping chip we made were accurate and sensitive Nine alleles of HLA A19 group antigen (A2901, A29XX, A3001, A3006, A30XX, A31, A32, A34 and A74) were accurately distinguished and its overall time was 2 5 hours Conclusion This proved that the DNA microarray technique was good for HLA A19 group genotyping and high resolution,high specificity, well reproducibility,intuitionistic and suitable for clinical application
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2004年第3期181-183,共3页
Chinese Journal of Laboratory Medicine
基金
国家十五科技攻关资助项目 ( 2 0 0 1BA80 1B0 3 )
