摘要
建立了化妆品中7种性激素(雌二醇、雌三醇、雌酮、睾酮、甲基睾酮、孕酮、己烯雌酚)同时测定的高效液相色谱法。先在试样中加入20g/L氢氧化钠溶液与油脂进行皂化反应,然后用二氯甲烷 乙酸乙酯(体积比为40∶1)混合液在酸性条件下(pH3)萃取,选择XTerraTMRP18色谱柱,以水 甲醇 乙腈(体积比为50∶32∶18)混合液为流动相,在波长230nm处检测。7种性激素分离良好并排除了样品中杂质峰的干扰,低、高浓度平均回收率范围为75 6%～97 8%;相对标准偏差为1 9%～7 2%;检出限为3 7～12ng。该方法可同时简便、准确地测定化妆品中的7种性激素。
An efficient method for analyzing seven sexual hormones in cosmetics, namely, estriol, estradiol-17β, estrone, testosterone, methyl-testosterone, progesterone and diethylstilbestrol by reversed-phase high performance liquid chromatography was developed. Samples were saponified with 20 (g/L) sodium hydroxide. The sexual hormones were then extracted with dichloromethane-acetic acetate (40∶1, v/v) under acidic conditions (pH 3, adjusted with 1 (mol/L) HCl ). An XTerra^(TM)RP_(18) column was employed and a mixture of water-methanol-acetonitrile (50∶32∶18, v/v) was used as mobile phase. The seven sexual hormones were detected at 230 nm. The average spiked recoveries for the seven sexual hormones ranged from 75.6% to 97.8% with relative standard deviations of 1.9% to 7.2%. The linear ranges of determination were from 5 to 50 (mg/L) with correlation coefficients of (0.999 9), and the limits of detection were from 3.7 to 12 ng. The method is suitable to simultaneously assay the seven sexual hormones in cosmetics with simplicity and accuracy in a single injection.
出处
《色谱》
CAS
CSCD
北大核心
2004年第3期267-269,共3页
Chinese Journal of Chromatography
关键词
反相高效液相色谱法
性激素
化妆品
reversed-phase high performance liquid chromatography
sexual hormones
cosmetics
