摘要
薄层等电聚焦电泳分析血清抗胰蛋白酶α_1-RT变异体,具有操作简便、分辨率高、重复性好等优点。α_1-AT的遗传缺陷与慢性阻塞性肺气肿、肝硬化等疾病的发生有关。本文报告利用薄层等电聚焦电泳技术对泸州地区362例入血清α_1-XT的遗传类型进行分析的结果。发现在受检人群中,MM型纯合子占97.79％(354/362),MS型杂合子占1.10％(4/362),MZ型杂合子占0.83％(3/362),其余0.28％(1/362)为ME型杂合子。此外,作者还对薄层等电聚焦电泳技术进行改良,使分辨率进一步提高。
The serum α_1-antitrypsin genotypses in Chinese population in Luzhou, Sichuan were deteted by a convenient, high-resolving and easilyreproducible method known as thin layer isoelectric focusing (TLIEF). The results have shown that most of the 362 subjects are of MM homozygotes(97.79％), whereas others are of MS heterozygotes (1.10％), MZ heterozygotes (0.83％) and ME heterozygotes (0.28％). In addition, the method was modified in several steps so that its resolving power could be further improved.
出处
《泸州医学院学报》
1992年第3期170-173,238-241,共4页
Journal of Luzhou Medical College
关键词
电泳
抗胰蛋白酶
遗传学
Thin layer isoelectric focusing electrophoresis
Alphal-antitrypsin
Genetics
Medical
