摘要
本文对a_1-抗胰蛋白酶异质体的检测方法(小扁豆凝集素亲合交叉免疫电泳法)进行改进。改进后的方法操作流程短、成本低、结果清晰、操作简便、不需预测a_1-抗胰蛋白酶总浓度,适合于临床广泛应用。本文用该法对原发性肝癌、转移性肝癌,其它器官癌肿、肝硬化及正常人血清中的a_1-抗胰蛋白酶异质体进行分析,发现小扁豆凝集素结合型a_1-抗胰蛋白酶百分比(LCA-R-AAT％)原发性肝癌组明显高于其它各组,以LCA-R-AAT(％)>25％为阳性标准,则原发性肝癌诊断的敏感性、特异性、准确性分别为80.6％、86.2％和84.3％,与小扁豆凝集素结合型甲胎蛋白联合检测,可使原发性肝癌诊断的敏感性提高至100％(P<0.05),特异性和准确性分别为82.8％和89.9％(P均>0.05),表明LCA-R-AAT(％)的检测对原发性肝癌的诊断及鉴别诊断有一定的价值。
A rapid and cheap method was developed to measure the reactivity of a-1-antitrypsin (AAT) with lens culinaris agglutinin (LCA). Using this method, we measured the percentages of LCA-reactive species of AAT (LCA-R-AAT (%)) in the sera of 89 persons from 5 groups (primary liver cancer (PLC), carcinoma metastatic to the liver (ML), cancer of other organs, liver cirrhosis and normal control), LCA-R-AAT(%) in PLC was higher than that in the other groups (P<0.05). The sensitivity, specificity and accuracy of LCA-R-AAT(%) in the diagnosis of PLC was 80.6%, 86.4% and 84.3% respectively. The percentage of LCA-reactive species of alpha-fetoprotein (LCA-R-AFP(%)) was measured in some of the persons, the sensitivity in the diagnosis of PLC by measuring the two parameters can rise to 100% (P<0.05), the specificity and the accuracy was 82.8% and 89.9% respectively (P> 0. 05) .
关键词
原发性
肝肿瘤
抗胰蛋白酶
异质体
lens culinaris agglutinin (LCA)
crossed immuno-affinity electrophoresis
LCA-reactive species of alpha-1-antitrypsin
LCA-reactive species of alpha-fetoprotein
primary livercancer
