摘要
肝细胞生成素 (HPO)具有复杂的生理功能 ,在睾丸中的高表达提示其在生殖活动中的重要性 ,而不仅局限于肝再生 .构建了酵母表达载体pGBKT7 HPO ,采用酵母双杂交系统 ,以HPO为诱饵蛋白 ,从人睾丸cDNA文库中寻找能够与HPO相互作用的蛋白质 .经过筛选、验证阳性克隆 ,并进行PCR、测序和序列比对 ,得到 4种相互作用蛋白质 :NADH脱氢酶 1、钠 /钾ATP酶β3亚基、磷脂酶Cδ1以及附睾分泌蛋白 .提示HPO可能参与了细胞的蛋白质合成 ,能量代谢等 .通过对候选蛋白的研究 ,为探讨HPO对睾丸组织细胞功能的调节机制提供了重要的线索 .
Hepatopoietin (HPO) has diverse functions in the regulation of cell growth and differentiation. Its high level expression in testis suggests that HPO may have important role for testicular physiological functions. Using HPO as 'bait', a yeast two-hybrid library screen of human testis was performed. By screening and selecting the positive colonies, retesting interactions in yeast, amplifying the AD/library inserts, sequencing and sequence comparing, four HPO-interacting proteins were identified: NADH dehydrogenase 1, Na(+)/K(+) ATPase beta-3 subunit (ATP1beta3), phospholipase C delta 1 and epididymal secretory protein. The significance of identification of these proteins may provide mechanistic insight into the biologic role of HPO in testis.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2004年第4期334-338,共5页
Progress In Biochemistry and Biophysics
基金
国家重点基础研究资助项目 (G19980 5 112 2 )
国家自然科学基金重点项目 ( 3 983 0 44 0 )
国家自然科学基金项目 ( 3 0 0 70 171)部分资助~~
