摘要
采用四甲基噻唑盐(M TT)法检验肝再生增强因子(ALR)对H epG 2细胞增殖作用;[3H]-T dR掺入测定细胞DNA合成;采用无机磷比色法测定细胞N a+,K+-ATPase的酶活力。结果表明:ALR通过促进H epG 2细胞DNA合成,使细胞增殖,并存在剂量效应正相关性(P<0.01);ALR对N a+,K+-ATPase酶活呈剂量时间依赖型影响;奎巴因可以通过抑制细胞N a+,K+-ATPase影响ALR对H epG 2细胞增殖促进作用。因此,N a+,K+-ATPase能参与调节ALR促进H epG 2细胞的增殖。
MTT assay and [^3H] -TdR incorporation were used to test the proliferation of HepG2 cell caused by augmenter of liver regeneration （ALR）. Inorganic phosphorus spectrophotometer was used to test the activities of the Na^＋,K^＋-ATPase. The results showed that ALR could promote the proliferation of HepG2 cell by increasing DNA synthesize rate, Na^＋,K^＋-ATPase activity was stimulated by ALR which depended upon the time and concentration. The proliferation effect which was caused by ALR were inhibited by ouabain, the Na^＋,K^＋-ATPase inhibitor. Na^＋,K^＋-ATPase contributed the regulation of ALR in proliferation of HepG2 cell.
出处
《广西农业生物科学》
CAS
CSCD
2006年第1期1-5,共5页
Journal of Guangxi Agricultural and Biological Science
基金
广东省自然科学基金资助项目(04102063)
