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猪心脏脂肪酸结合蛋白基因PCR-RFLP分子标记研究 被引量:11

Study on PCR-RFLP molecular marker of pig heart fatty acid-binding protein gene
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摘要 利用PCR-RFLP分子标记技术,检测了杜洛克、长白、大白、内江、荣昌、汉江黑、汉白、八眉和野猪共计265头猪心脏脂肪酸结合蛋白基因5′上游区和第二内含子区的遗传变异.结果表明,在Hinfl-RFLP位点上,上述猪种和野猪均存在多态性,等位基因H的频率分别为0.750 0,0.718 8,0.916 7,0.333 3,0.125 0,0.690 9,0.116 7,0.850 0和0.937 5;除汉江黑猪(P<0.05)和野猪(P<0.01)外,其余的猪种基因频率和基因型频率都处于Hardy-Weinderg平衡状态(P>0.05);大白、八眉、汉江黑、汉白和野猪表现为低度多态(PIC<0.25),杜洛克、长白、内江和荣昌猪为中度多态性(0.25<PIC<0.5);而在HaeⅢ-RFLP和MspI-RFLP位点上,仅内江猪、荣昌猪、汉江黑猪和八眉猪为单态.
出处 《生物技术通讯》 CAS 2004年第2期124-127,共4页 Letters in Biotechnology
基金 国家自然科学基金
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参考文献15

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二级参考文献1

  • 1吴桢方.华中农业大学博士学位论文[M].-,1998..

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