摘要
目的 观察门静脉主干缩窄法制备的大鼠门静脉高压症模型的维持时间以及脾脏的动态变化 ,为该模型是否可同时作为门静脉高压症脾亢模型使用提供依据。方法 雄性SD大鼠70只 ,其中 60只先采用门静脉主干缩窄法制备门静脉高压症模型 ,再按预定要观察的时间随机分为制模后 1、2、4、8、12、2 4周 6个实验组 ,每组 10只。另 10只作为正常组。各组在预定观察的时间进行门静脉压力、血常规、脾指数、脾横轴径测定 ,并作骨髓病理学检查。结果 1～ 8周实验组 ,门静脉压力较正常组均显著增高 ,分别为 (1.98± 0 .18)、(1.96± 0 .17)、(1.80± 0 .18)、(1.41± 0 .2 0 )kPa ,正常组 (0 .92± 0 .16)kPa(P均 <0 .0 5 ) ;脾指数较正常组均显著增大分别为 (4 .5 5± 0 .71)、(4 .0 6± 0 .68)、(3 .81± 0 .5 6)、(3 .5 3± 0 .41)mg/g ,正常组 (2 .69± 0 .3 5 )mg/g ,(P均 <0 .0 5 ) ;脾横、轴径较术前均显著增长 ,横径在术后为 (1.0 2± 0 .0 8)、(0 .96± 0 .0 9)、(0 .90± 0 .0 7)、(0 .85± 0 .0 9)cm ,术前为 (0 .75± 0 .0 7)、(0 .76± 0 .0 8)、(0 .78± 0 .0 8)、(0 .76± 0 .0 8)cm ;轴径在术后为 (4 .5 3± 0 .5 1)、(4 .2 2± 0 .60 )、(4 .18± 0 .3 1)、(3 .91± 0 .2 0 )cm ,术前为 (3 .72±
ObjectiveTo explore how long the condition of portal hypertension can persist by portal vein stenosis in Sprague-Dawley rats and the splenic dynamical changes,and investigate that if this model can be used in the research of hypersplenism due to portal hypertension or not.Methods70 Sprague-Dawley male rats were randomly divided into one normal group (10 rats) and experiment group (60 rats).In the 60 rats of experiment group,the model of portal hypertension was created by portal vein stenosis and then according to the anticipated observation period (1,2,4,8,12,24 weeks),the total 60 rats were randomly divided into 1W-group,2W-group and 24W-group (10 rats per group).The normal group only did sham operation.The portal pressure,blood routine test,spleen index,dimensions of spleen,and the pathology of spleen and bone marrow of each group were examined at anticipated observation period.ResultsIn 1W-8W-experiment group,portal vein pressure (1.98±0.18,1.96±0.17,1.80± 0.18,1.41±0.20 kPa,respectively) and spleen index (4.55±0.71,4.06±0.68,3.81±0.56,3.53± 0.41 mg/g respectively) were increased significantly (all P <0.05) as compared with normal group (portal pressure:0.92±0.16 kPa,spleen index:2.69±0.35 mg/g).The dimension of spleen druing the experiment of 1W-8W was also increased significantly after the operation (transverse diameter:postoperative 1.02± 0.08,0.96±0.09,0.90±0.07,0.85±0.09 cm,preoperative 0.75±0.07,0.76±0.08, 0.78±0.08,0.76±0.08 cm;axis diameter:postoperative 4.53±0.51,4.22±0.60,4.18±0.31, 3.91±0.20 cm,preoperative 3.72±0.41,3.65±0.29,3.68±0.33,3.57±0.36 cm,P <0.05).Platelets were decreased in the 2W-8W-experiment groups but had no significant difference ( P >0.05) and the hemoglobin,erythrocytes and leucocytes had no difference between experimental and normal groups ( P >0.05).The score of bone marrow hyperplasia had no obvious difference among the groups.ConclusionPortal vein stenosis can successfully cause portal hypertension in rats and the condition of portal hypertension can last 8 weeks after operation but not develop hypersplenism,so it can't be used as a model of hypersplenism due to portal hypertension.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2004年第4期433-435,共3页
Chinese Journal of Experimental Surgery
基金
陕西省科技攻关项目 (2 0 0 3K1 0 G73)
