摘要
背景:严重脾破裂患者可通过自体脾组织大网膜内移植挽救脾脏的功能,移植脾组织是否受神经调控目前尚不清楚。生长相关蛋白(growthassociatedprotein,GAP-43)是一种神经系统特异性蛋白质,通过检测移植脾组织内GAP-43以了解其神经再生情况。目的:研究自体脾组织移植术后不同时相再生脾组织中GAP-43mRNA的表达,揭示GAP-43+神经在自体移植脾组织中的再生规律。设计:随机对照实验。单位:一所大学的外科应用解剖与手术学教研室。材料:实验于2002-09/2003-07在第三军医大学外科应用解剖与手术学教研室进行。选用Wistar大鼠120只,随机分为实验组和假手术组(对照组)制作动物模型。术后两组动物在同等条件下饲养,分别于15,30,60,90,120,180d,取脾组织进行观察。方法:采用Tripure试剂按常规方法提取组织中总RNA。采用M-MLV反转录试剂盒将总RNA反转录cDNA。凝胶图像分析定量测定GAP-43mRNA。主要观察指标:①反转录-聚合酶链反应检测结果。②图像分析结果。结果:脾组织移植手术后30d,自体移植脾组织中出现GAP-43mRNA;手术后90d达到高峰;手术后120～180d,移植脾组织中GAP-43mRNA逐渐接近正常脾组织。结论:GAP-43mRNA在自体移植脾组织中有表达,提示移植脾组织中有神经纤维的再生。
BACKGROUND:The patients with severe spleen rupture can save their spleen functions by auto transplantation of the spleen tissues in the greater omentum.Whether the transplanted spleen tissues are regulated by nerves or not is still unclear.The growth associated protein,GAP 43,is a specific protein of the nervous system,and by testing the GAP 43 in the transplanted spleen tissues,nerve regeneration can be understood.OBJECTIVE:To study the mRNA expression of the GAP 43 in the regenerated spleen tissues at different phases after the auto transplantation of the spleen tissues, and to find the regeneration regularity of the GAP 43+ nerves in the auto transplanted spleen tissues.DESIGN:A randomized and controlled experimentSETTING:A teaching and research office of the surgical applied anatomy and operative surgery department in a universityMATERIALS:The study was done in the Surgical Applied Anatomy and Operative Surgery Department of the Third Military Medical University of Chinese PLA from September 2002 to July 2003.A total of 120 Wistar mice were chosen.The mice were randomly divided into the experiment group and pseudo operation group(control group) to make animal models.After the operation, mice of the two groups were fed under the same circumstances.The spleen tissues were respectively taken at the 15th, 30th, 60th, 90th, 120th and 180th days after the operation for the study.METHODS:The general RNA was extracted from the tissues using the Tripure agent by the routine method.The general RNA was reverse transcribed into cDNA using the M MLV reverse transcription kit.The mRNA of the GAP 43 was quantitatively measured using the gel pattern analysis.MAIN OUTCOME MEASURES:① The result of the reverse transcription polymerase chain reaction(RT PCR)measurement;② The result of the pattern analysis.RESULTS:Totally 30 days after the transplantation of the spleen tissues,the mRNA of the GAP 43 was found to be expressed in the auto transplanted spleen tissues.Ninety days after the operation, the expression reached the peak level.Totally 120 to 180 days after the operation the amount of the mRNA of the GAP 43 in the transplanted spleen tissues gradually got close to that in the normal spleen tissues.CONCLUSION:The expression of mRNA of the GAP 43 in the auto transplanted spleen tissues suggests the regeneration of the nervous fiber in the transplanted spleen tissues.
出处
《中国临床康复》
CSCD
北大核心
2005年第17期236-237,F003,共3页
Chinese Journal of Clinical Rehabilitation
