摘要
目的 :研究鸦胆子油乳诱导白血病细胞 (U937细胞 )凋亡及凋亡过程中caspase 3的变化及其意义。方法 :MTT法检测鸦胆子油乳对U937细胞增殖的抑制率 ;通过细胞形态、DNA片段化电泳、流式细胞术 (FCM )检测细胞凋亡 ;应用酶联反应法检测caspase 3活性。 结果 :在 31.2 5～ 1 0 0 0 .0 0mg/L浓度下鸦胆子油乳抑制U937细胞的增殖 ,IC5 0值为 2 14mg/L ,并呈现剂量 时间 效应关系 ;5 0 0mg/L鸦胆子油乳处理U937细胞 ,出现典型的细胞凋亡形态特征和DNA梯形带。流式细胞术检测显示 12 5、2 5 0、5 0 0mg/L鸦胆子油乳诱导U937细胞凋亡 ;在诱导细胞凋亡过程中Caspase 3活性明显增高 ,且随作用时间延长 ,活性增加。 结论 :鸦胆子油乳在体外能够诱导U937细胞凋亡 ,诱导细胞凋亡可能是鸦胆子油乳抗白血病作用的机制之一 ,caspase 3活化参与了鸦胆子油乳诱导U937细胞凋亡的调控。
Objective:To study the variation and significance of Caspase-3 activity in the fructus bruceae emulsion inducing human leukemic cell (U937) apoptosis.Method:Inhibition of proliferation was measured by MTT assay. The cell apoptotic were examined by microscope, DNA fragmentation electrophoresis and flow cytometry (FCM). The activity of caspase-3 was detected by enzyme-linked immunosorbent assay.Result:The proliferation of U937 cells were significantly inhibited by 31.25～1000 μg/ml of fructus bruceae emulsion and showed a dose and time-dependent relationships. The IC50 value was 214 μg/ml. Typical morphological changes and DNA ladder were observed. while treated with fructus bruceae emulsion in125,250 and 500 μg/ml for 24 h,48 h and 72 h, the apoptotic ratios observed by FCM showed significant differences comparing with the control respectively (P< 0.01).The activity of caspase-3 was significant higher in the apoptosis than in control cells.Conclusion:Fructus bruceae emulsion could induce apoptosis of U937 cells in vitro, which may be one of the anticancer mechanisms.Caspase-3 activation mar participate in the processes of the apoptosis of U937 cells induced by fructus bruceae emulsion.
出处
《临床血液学杂志》
CAS
2004年第3期154-156,共3页
Journal of Clinical Hematology
