摘要
目的探讨鸦胆子油乳注射液对人脑胶质瘤细胞增殖的影响及其作用机制。方法体外培养人脑胶质瘤细胞SKMG-4;利用四甲基偶氮唑盐(MTT)比色法检测鸦胆子油乳注射液对SKMG-4增殖的抑制作用;利用流式细胞术(FCM)分析药物处理前后SKMG-4的细胞周期变化;应用免疫组化检测药物处理前后bcl-2表达情况。结果 MTT比色法显示鸦胆子油乳注射液对SKMG-4细胞增殖的抑制作用呈剂量依赖性,当浓度为5 g/L时,对SKMG-4细胞增殖的抑制率达到72.1%±2.2%(P<0.05)。流式细胞仪分析显示,用5 g/L鸦胆子油乳注射液培养48 h,细胞周期中G1期细胞所占比例增高,S期所占比例降低。免疫组化显示随着鸦胆子油乳注射液作用剂量的增加,bcl-2基因的表达被明显抑制。结论中药鸦胆子油乳注射液能通过调控细胞周期,抑制bcl-2凋亡基因的表达抑制人脑胶质瘤细胞SKMG-4的增殖。
Objective To explore the effect of brucea javanica oil emulsion on proliferation of human glioma cells and its mechanism.Methods Human SKMG-4 glioma cells were cultured.3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）colorimetric assay was used to measure the inhibitory effect of brucea javanica oil emulsion on proliferation of SKMG-4 golima cells.Flow cytometry（FCM） analysis was used to analyze the cycle changes of SKMG-4 glioma cells before and after drug treatment.Immunohistochemical staining was used to detect the expression of bcl-2 before and after drug treatment.Results MTT demonstrated that brucea javanica oil emulsion could dose-dependently inhibit proliferation of SKMG-4 glioma cells with an inhibition rate of 72.1%±2.2% when the concentration of brucea javanica oil emulsion was 5 g/L（P0.05）.Flow cytometry analysis showed that the percentage of G1 phase was increased,and that of S phase was decreased after cultured in 5 g/L brucea javanica oil emulsion for 48 h.The immunohistochemistry showed that expression of bcl-2 was remarkably inhibited with the increase of dose of brucea javanica oil emulsion.Conclusion Brucea javanica oil emulsion can significantly inhibit the proliferation of SKMG-4 glioma cells by regulating the cycle progression and inhibiting the expression of bcl-2 gene.
出处
《中华神经外科疾病研究杂志》
CAS
2011年第1期38-40,共3页
Chinese Journal of Neurosurgical Disease Research
