摘要
背景:星形细胞对神经元有提供营养、支持及调节突触活性作用,但它对神经元发育的影响还尚不清楚。目的:探讨体外培养的SpragueDawley大鼠大脑皮质星形细胞对PC12神经元突起生长发育的作用。设计:完全随机设计,对照实验研究。方法:以培养的星形细胞与PC12神经元按不同细胞数目比例(50∶1～1∶1)共同培养,并用其制备的条件培养液培养PC12细胞。主要观察指标:用快速灵敏的MTT比色法测定PC12神经元的细胞活力,用光学相差显微镜观察PC12细胞形态学变化。结果:①星形细胞条件培养液可增强PC12细胞活力(MTT测定的A值由0.255±0.012提高到0.510±0.036,P<0.001),却不能促使PC12神经元突起的生出。②当将星形细胞与PC12细胞按30∶1～1∶1的比例共同培养时,既可提高PC12细胞折光性和光晕又可促使其突起的生长;但按50∶1～40∶1的比例共同培养时,只观察到提高PC12细胞折光性和光晕,而无促使其突起生长发育的作用。结论:PC12神经元细胞活力的提高与星形细胞分泌到条件培养液中的可溶性因子有关,而PC12神经元突起生长发育可能是和与星形细胞的直接接触以及二者的细胞数目比有关。
BACKGROUND:Although astrocytes are kown to provide structural and trophic supp ort to neurons and modulate synaptic activity,their role is far from being compl etely understood. OBJECTIVE:To investigate effects of cultured astrocytes from Sprague-Dawley r at cerebral cortex on the neurite development of PC12 cells derived from rat phe ochromocytoma. DESIGN:Completely randomized controlled trial. METHODS: PC12 cells were co-cultured with astrocyte according to different as trocytes/neurons ratio(50∶1-1∶1),or cultured with serum-free conditioned med ium of astrocytes(ACM). MAIN OUTCOME MEASURES:The vitality of PC12 cells was measured by sensitive MTT method and their morphologic features were observed by Olympus light microscope . RESULTS:When PC12 cells were cultured with ACM,compared with the control group , the vitality of PC12 cells was increased significantly(0.255±0.012 vs 0.510± 0.036,P< 0.001) ,but the neurite growth was not obvious in the experimental gro up.When PC12 cells were co-cultured with astrocyte in the ratio of 30∶1-1∶1, not only was the vitality of PC12 cells enhanced, but also the neurite-outgrowt h of PC12 was observed.However,when PC12 cells were co-cultured with astrocyte in proportion of 50∶10-40∶1,the vitality of PC12 cells was also enhanced,but the neurite-outgrowth of PC12 was not found. CONCLUSION:This study suggested enhancement of PC12 cell-vitality was mediate d by soluble factors produced by astrocytes,while activity of the neurite-promo ting was associated with cell-cell contact and with the ratio of two cells.
出处
《中国临床康复》
CSCD
2004年第13期2570-2571,F003,共3页
Chinese Journal of Clinical Rehabilitation
基金
广东省自然科学基金(020016)~~
