摘要
本研究从大鼠大脑皮质分离、纯化星形胶质细胞,再经培养后收集星形胶质细胞的无血清条件培养液。用盖玻片培养法与快速自动比色微量分析法研究了星形胶质细胞条件培养液对小脑皮质神经元生存以及神经元活力的影响。发现星形胶质细胞条件培养液能够明显提高小脑皮质神经元的体外存活率,增强神经元的活力。表明星形胶质细胞具有神经营养性作用。
The rat cerebral cortical astrocyte culture was prepared and the conditioned medium was collected to measure its neurotrophic effect on cerebellar cortical neurons in vitro by covers-lip-culture method as well as the automated colorimetric microassay technique. It was found that the astrocyte conditioned medium could obvious1y support the neuronal survival of cerebellar cortical neurons, and enhance the neuronal vigour. These results further confirm the prev-ious viewpoint that astrocytes may have neurotrophic activity.
出处
《细胞生物学杂志》
CSCD
1997年第1期23-26,51,共4页
Chinese Journal of Cell Biology
基金
卫生部科学研究基金
广东省博士后科学研究经费部分资助
