摘要
目的用高效液相色谱法同时测定中药蛇床子中蛇床子素和欧前胡素的含量。方法采用KromasilC8(2 5 0mm× 4 6mm ,5 μm)为色谱柱 ,以甲醇 水 (体积比 6 5∶35 )为流动相的HPLC法 ,检测波长为 310nm ,流速为 0 8mL·min-1,柱温为室温。结果以醋酸氟轻松为内标 ,蛇床子素和欧前胡素分别在 13～ 2 0 8mg·L-1和 8～ 12 8mg·L-1内 ,线性关系良好 (r≥ 0 9998) ;平均回收率分别为 98 2 %和 97 5 % ;RSD分别为 1 7%和 2 0 % (n =9)。结论本法简便快速 ,回收率及重现性良好 。
Objective To develop a method for determination of osthole and imperatorin in Fructus Cnidii by RP-HPLC.Methods Chromatography was performed using a Kromasil C 8( 250 mm× 4.6 mm, 5 μm) column with mobile phase of methanol-water (65∶35).The monitoring wavelength was 310 nm.The flow rate was 0.8 mL·min -1 and the column temperature was room temperature.fluocinonide was served as an internal standard.Results A good linearity between peak area ratio of the common peak to the internal standard and the concentration was found in the range of 13～ 208 mg·L -1 for osthole and 8～ 128 mg·L -1 for osthole ( r>0.999 8) and imperatorin ( r>0.999 9),respectively.The average recoveries were 98.2% (n=9,RSD=1.7%) and 97.5%(n=9,RSD=2.0%) for osthole and imperatorin,respectively.Conclusion The method is simple,rapid and with satisfactory recoveries and good reproducibilities.It can be used to control the quality of Fructus Cnidii.
出处
《沈阳药科大学学报》
CAS
CSCD
2004年第3期201-204,共4页
Journal of Shenyang Pharmaceutical University
