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口蹄疫病毒泛亚株全衣壳基因克隆和序列结构分析 被引量：2

The cloning and sequence analysis of the capsid gene (P1) of foot-and-mouth disease virus O/PanAsia
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摘要 应用RT PCR和nPCR实现了口蹄疫病毒O/PanAsia/10株的全衣壳基因的克隆和序列测定.对核苷酸和氨基酸的序列分析表明,与经典O型毒株比较存在较大变异.其中4种结构蛋白的变异顺序是VP1>VP3>VP2>VP4.P1基因G+C值约55%.全衣壳蛋白相对分子质量约8 0×104.主要结构蛋白VP1呈碱性,等电点约9 5. A reverse transcription polymerase chain reaction (RT-PCR) and nested PCR (nPCR) is described that amplified the genes encoding the capsid proteins VP1-4 of the foot-and-mouth disease (FMD) virus (O/PanAsia/10.) The sequences of the antigenically relevant capsid proteins VP1-3 were compared with those of previous field viruses and found to be highly variable by phylogenetic analysis as following:VP1>VP3>VP2. The G+C value of P1 gene is about 55% and the size of the whole capsid protein is about 8.0×10~4. The major structural protein VP1 is basic and its isolectric point is pH 9.5, much higher than those of VP2, VP3 or VP4.
作者 田纯见 曹永长 毕英佐
机构地区 华南农业大学动物科学学院
出处 《华南农业大学学报》 CAS CSCD 北大核心 2004年第2期89-92,共4页 Journal of South China Agricultural University
关键词 口蹄疫病毒 泛亚株 衣壳蛋白 基因克隆 序列分析 RT-PCR nPCR 口蹄疫 foot-and-mouth disease virus capsid protein gene cloning gene sequence analysis
分类号 S852.659.6 [农业科学—基础兽医学] S855.3 [农业科学—临床兽医学]
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参考文献13

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共引文献2

同被引文献20

  • 1吴健敏,任兆钧,余兴龙,张念祖,涂长春.猪瘟病毒E2蛋白重组T4噬菌体的构建及免疫学特性[J].中国兽医学报,2004,24(6):521-524. 被引量：5
  • 2余晓岚,肖少波,方六荣,胡梦雨,严琳,董晓辉,陈焕春.口蹄疫病毒P1基因在大肠杆菌中的高效表达及其生物活性的初步分析[J].生物工程学报,2005,21(1):163-166. 被引量：18
  • 3刘铀,毕英佐,曹永长,马静云,肖俊芳.新城疫病毒HN蛋白球状头部在T4噬菌体衣壳表面的展示[J].中国兽医学报,2005,25(4):346-349. 被引量：2
  • 4韩松,金宁一,鲁会军,郑敏,尹革芬,葛淑敏,金扩世.O型口蹄疫病毒VP1基因的克隆及其在重组杆状病毒中的表达[J].中国兽医学报,2006,26(1):42-44. 被引量：1
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华南农业大学学报
2004年 第2期

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