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口蹄疫病毒结构蛋白P1基因植物表达载体构建及鉴定 被引量:1

Construction and Identification of Plant Expression Vector Foot and Mouth Disease Virus (FMDV) P1
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摘要 通过RT-PCR克隆了口蹄疫病毒(FMDV)全长P1基因,然后与植物的表达盒融合构建了重组质粒pBI131SP1、pBIP1和pBIAP1,并将质粒转化到根癌农杆菌LBA4404和EHA105中,获得了植物双元表达载体。 FMDV-P1,P1 gene was cloned by RT-PCR and pBI131SP1,pBIP1 and pBIAP1vector was constructed, then the vector was transformed into Agrobacterium tumefaciens (LBA4404 and EHA105). Double expressing plant vector was obtained.
出处 《吉林农业大学学报》 CAS CSCD 北大核心 2004年第1期23-26,31,共5页 Journal of Jilin Agricultural University
基金 863"资助项目(2001AA213071) 吉林省科技厅资助项目(20030552-4)
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