摘要
通过RT-PCR克隆了口蹄疫病毒(FMDV)全长P1基因,然后与植物的表达盒融合构建了重组质粒pBI131SP1、pBIP1和pBIAP1,并将质粒转化到根癌农杆菌LBA4404和EHA105中,获得了植物双元表达载体。
FMDV-P1,P1 gene was cloned by RT-PCR and pBI131SP1,pBIP1 and pBIAP1vector was constructed, then the vector was transformed into Agrobacterium tumefaciens (LBA4404 and EHA105). Double expressing plant vector was obtained.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2004年第1期23-26,31,共5页
Journal of Jilin Agricultural University
基金
863"资助项目(2001AA213071)
吉林省科技厅资助项目(20030552-4)