摘要
目的:探讨褪黑素对大鼠肝再灌注损伤的影响作用及其机制方法:150只健康♂Wistar大鼠(质量190-210 g,6-7周龄),随机分为褪黑素处理组(Mel)、酒精溶媒对照组(Alc)和生理盐水对照组(NS).建立肝缺血再灌注损伤模型,缺血均为60 min,之后每组分别按再灌注后30 min、1、6、12、24 h采集标本.M组(20 mg/kg)于缺血前30 min腹腔注射melatonin;A组采取与Mel组相同浓度的酒精液,N 组则注射同比例的生理盐水.测定血清丙氨酸氨基转移酶(ALT),肝组织中超氧化物岐化酶(SOD)及肝组织过氧化的终产物-丙二醛(MDA),对肝组织进行HE染色及ICAM- 1免疫组化染色. 结果:Mel组在再灌注后各时点的ALT均显著低于Alc及NS 对照组(aP<0.05),且Alc组与NS组相比无显著性差异. Mel组在再灌注后6、12、24 h时点的MDA显著低于Alc 及NS对照组(aP<0.05),且各时点内Alc组与NS组相比无显著性差异.Mel组在再灌注后12、24 h时点的SOD显著高于Alc及NS对照组(cP<0.05),且各时点内Alc组与NS组相比无显著性差异.Mel组在再灌注后各时点ICAM- 1染色的阳性细胞率均显著低于Alc组和NS组(aP<0.05), 且每时点内的Alc组与NS组相比无显著性差异. 结论:外源性Mel可以抑制再灌注后血清丙氨酸氨基转移酶(ALT),增加肝组织超氧化物歧化酶(SOD)、的活性,减少肝组织MDA的浓度,抑制肝组织ICAM-1蛋白的表达, 对缺血再灌注肝损伤有明确的保护作用.
AIM: To investigate the effect of melatonin (Mel) on liver ischemia reperfusion (I/R) injury in rats. METHODS: 150 male Wistar rats (190-210 g, 6-7weeks age) were divided into three groups at random: Mel exposure group, alcohol solvent control group and saline control group. The left branches of portal vein, hepatic artery, hepatic duct were blocked up for 60 min and then opened to establish liver I/R I models in rats. In each group, samples were collected in 0.5, 1, 6, 12, and 24 h after reperfusion respectively. 20 mg/kg of Mel was injected peritoneally in rats 30 min before experimentation in Mel exposure group. The duplicate concentration of alcohol and the same volume of saline were injected in control group as a substitution. Serum alanine aminotransferase (ALT) by auto biochemical analyzer, and superoxide dismutase (SOD) and terminal productions of lipid peroxidationin (MDA) in liver tissue were measured. Pathological changes in liver and immunohistochemical straining of ICAM-1 were determined with optical microscope. RESULTS: The level of ALT measured in various time after reperfusion in Mel group was totally significantly lower than that in alcohol and saline control groups (P <0.05). The level of MDA measured in 6 h, 12 h, and 24 h after reperfusion in Mel group was significantly lower than that in alcohol and saline control groups (P <0.05). The level of SOD measured in 12, 24 h after reperfusion in Mel group was significantly higher than that in alcohol and saline control groups (P <0.05). The expression level of ICAM-1 (%) measured in various time after reperfusion in Mel group was significantly lower than that in alcohol and saline control groups (P<0.05). CONCLUSION: Exotic Mel inhibits the activities of ALT, increases activities of superoxide dismutase (SOD), and decreases the cumulation of MDA in liver reperfusion tissue and expression of ICAM-1 in liver reperfusion tissue. Therefore, it can improve the hepatic function after reperfusion and plays a definitely protective role in liver I/R.
出处
《世界华人消化杂志》
CAS
2004年第4期880-885,共6页
World Chinese Journal of Digestology
基金
辽宁省自然科学基金资助项目
No.619025~~
