摘要
以致病性大肠杆菌为宿主菌 ,从发病鸡场土壤、鸡粪中分离出 1 0余株噬菌体 ,并从中筛选出噬菌斑大且裂解快的 3株 ,对其生物学特性进行了研究。结果表明 :3株噬菌体在 5 0℃处理 60min或在 60℃处理 2 0min后仍具较高活性 ,且耐受pH范围广泛 ;培养基中加入Mg2 + 或Ca2 + 可促进噬菌体的裂解 。
More than ten bacteriophage of E.coli were isolated from the soil and the dung of the fowl-run, then three of named bacteriophage A, C, D which lysis E.coli virulently were selected to investigate biological characterizations. The results showed that high activities were obtained after the phages incubated at 50℃ for 1 h or 60℃ for 30 min. The phages could be alive at the range of pH from 4 to 12, Ca 2+ or Mg 2+ added to the medium could stimulate the lysis of phages. However, the formation of the plaque could be inhibited obviously by adding sodium citrate to the medium.
出处
《微生物学通报》
CAS
CSCD
北大核心
2004年第2期10-13,共4页
Microbiology China
基金
山东省科委资助项目 (No 0 12 10 0 10 4 )
关键词
致病性大肠杆菌
噬菌体
生物学特性
Virulent Escheria coli, Bacteriophage, Biological characterization
