摘要
研究了用固相萃取和高效液相色谱法测定烟草样品中的叶黄素和β 胡萝卜素;烟草样品中的叶黄素和β 胡萝卜素用90%的丙酮震荡萃取,然后用WatersSep Park C18固相萃取小柱预分离和富集,以WaterNova Pak C18色谱柱为固定相,(1+1)甲醇异丙醇溶液 水梯度洗脱为流动相,二极管矩阵检测器检测测定。该方法标准回收率为96%～104%,RSD%为1.26%～2.43%;用于烟草中叶黄素和β 胡萝卜素的测定,结果满意。
Determination of β-carotene and lutein from tobacco was studied by solid phase extraction and HPLC. Samples were extracted with 90% acetone by oscillating, enriched by solid phase extraction on a Sep-Pak-C(18) chromatogram column (3.9 ～150?mm), isolated by a Waters Nova-Pak-C(18)column eluting with gradient mixture of methanol-isopropanol (1∶1) -water, and then determinated. The detection limit was 40 ng/ml for both β-carotene and lutein, with recovery rate as 96%～104% and RSD as 1.26%～2.43%. The method was applied to the determination of β-carotene and lutein from tobacco with good results.
出处
《云南化工》
CAS
2004年第2期39-41,共3页
Yunnan Chemical Technology
关键词
烟草
叶黄素
Β-胡萝卜素
测定
高效液相色谱法
high performance liquid chromatography
lutein
β-carotene
tobacco
solid phase extraction
