摘要
利用本研究室制备的抗α-突触核蛋白(α-Synuclein,α-SYN)单克隆抗体作为研究工具,在一种低氧预适应小鼠模型上研究了重复急性低氧对大脑皮层中α-SYN表达的影响。Western blot分析结果表明,小鼠在经过重复急性低氧刺激后,皮层脑组织内α-SYN蛋白含量呈现规律性的变化,表现为第一次急性低氧后明显增加,而经过重复低氧第4次后则回落,接近正常水平;免疫组化染色结果显示,α-SYN免疫阳性物质除存在于神经元的末梢外,还存在于某些神经元的核中。分析大脑皮质细胞核呈α-SYN免疫阳性的神经元的密度发现,核呈α-SYN阳性的神经元密度也在第一次急性低氧后明显增加,而经过重复低氧第4次后回落。以上结果提示,重复急性低氧能够改变α-SYN在脑内的表达水平以及α-SYN核阳性神经元的数量。这种变化的机制和生理意义有待于进一步探讨。
An anti-α-synuclein (α-SYN) monoclonal antibody produced in our laboratory was used to investigate the effect of repeated acute hypoxic treatments on the expression of α-SYN in the mouse cerebral cortex. Western blot analysis showed that the expression levels of α-SYN in the cortex changed accordingly upon hypoxic exposure times, as that the α-synuclein level significantly increased after the first hypoxic exposure and then dropped down to the background level after the fourth hypoxic exposure. Immunohistochemical staining revealed that the -SYN-immunopositive substance was localized not only in the nerve endings, but also within the nuclei of some neurons. The cell density of the neurons with α-SYN immunopositive nuclei was increased significantly after the first hypoxic exposure but returned back to control levels after the fourth hypoxic exposure. Our results indicate that both of the α-SYN expression level in the brain and the number of the neurons with α-SYN positive nuclei are affected by the repeated acute hypoxic treatments and that this modification is hypoxic time-dependent. The mechanism and the physiological significance underlying these changes need to be further investigated.
出处
《生理学报》
CAS
CSCD
北大核心
2004年第2期263-268,共6页
Acta Physiologica Sinica
基金
This work was supported by the National Basic Research Priorities Programme (G2000077005)
National Natural Science Foundation of China (No.3027048) and Beijing Natural Science Foundation (No.7022011).
