摘要
目的:探讨恶性组织细胞增生症瘤细胞的属性,EB病毒感染的情况及其临床病理特点。方法:收集33例以往诊断为恶组的尸检病例及相关临床资料,并利用组织芯片技术将不同组织集成在一张切片上。免疫组化采用LsAB法。一抗选用CD4、CD8、CD20、CD45RO、CD56、CD3ε、CD30、CD68、TIA-1及GranzymeB。DNA-RNA原位杂交检测EB病毒编码的小分子mRNA。结果:33例恶组中,排除不符合传统恶组诊断标准的4例,选择其中29例作为研究对象。检测结果示29例中有28例属外周T细胞淋巴瘤,其中皮下脂膜炎样T细胞淋巴瘤3例、肠道T细胞淋巴瘤1例,间变性大细胞淋巴瘤1例;16/28例为EBER1/2阳性;余1例为恶组样B细胞性血管内淋巴瘤。结论:恶性组织细胞增生症是一组异质性淋巴细胞增生性疾病。绝大多数瘤细胞来源于细胞毒性,T细胞或NK细胞(28/29),少数来源于B淋巴细胞(1/29),未发现组织细胞源性的肿瘤。
Objective: 33 autopsy cases of previously diagnosed malignant histiocytosis were investigated to the tumor cell lineage, Epstein-Barr virus infection and clinicopathological features. Methods: With tissue microarray technique, different tissues from paraffin embedded tissue blocks of each case were integrated into one paraffin block. Then according routing method, multitissue sections were prepared for next immunostaining and in situ hybridization. Using LsAB method, immunohistochemical stainings were performed. The applied antibodies were as follows: CD4, CD8, CD20, CD45RO, CD56, CD3ε, CD30, CD68, TIA-1 and granzyme B. A DNA-RNA in situ hybridization was used for detection of mRNA encoded by EBV(EBER1/2). Results: 4 cases were deleted from the 33 cases according to the results of immunostainings. In the remaining 29 cases, 28 cases were peripheral T-cell lymphoma, in which 3 were subcutaneous panniculitis like T cell lymphoma, 1 was intestinal T cell lymphoma, and 1 was anaplastic large cell lymphoma. There were 16 of 28 T cell lymphomas with EBER1/2 positive reactions. There was one case of intravascular large B-cell lymphoma. Conclusions: In cell lineage, so called malignant histiocytosis is a group of heterogeneous disorders. In our study most cases represented cytotoxic cell lymphomas(28/29), only one came from B cell, and none of them from monocyte/macrophage.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2004年第6期301-305,共5页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金(编号:39770298)
四川省科技厅重点课题基金(01SG013-103)
美国中华医学基金(CMB)部分资助(00-722)
