摘要
目的 :探讨转化生长因子 -β1(TGF β1)信号通路失活与直肠癌恶变过程的关系。方法 :应用免疫组化SP法检测 2 0例正常直肠黏膜、13例直肠腺上皮不典型增生及 61例不同分化程度直肠腺癌中TGF β1、转化生长因子 βⅡ型受体(TβRⅡ )、胰腺癌缺失基因 4(Smad4)和血管内皮生长因子 (VEGF)蛋白的表达。结果 :TGF β1在低分化 (13 /18)、中分化(17/2 3 )和高分化 (11/2 0 )组中的阳性表达明显高于不典型增生组 (3 /13 ) ,差异有统计学意义 ,P <0 0 5 ;且与淋巴结侵犯明显相关 ,P <0 0 1。TβRⅡ在正常直肠黏膜 (15 /2 0 )及不典型增生组 (7/13 )中的阳性表达明显高于低分化 (3 /18)、中分化(4 /2 3 )和高分化 (4 /2 0 )组 ,P <0 0 5。低分化 (13 /18)、中分化 (16/2 3 )腺癌有Smad4的缺失表达 ,与高分化 (7/2 0 )、不典型增生组 (4 /13 )及正常直肠黏膜(6/2 0 )的缺失表达差异有统计学意义 ,P <0 0 5。VEGF在低分化 (12 /18)和中分化 (16/2 3 )组中的阳性表达明显高于高分化 (5 /2 0 )及不典型增生组 (3 /13 ) ,差异有统计学意义 ,P <0 0 5。TGF β1与VEGF的表达呈正相关 ,Smad4与TβRⅡ的表达呈正相关 ,Smad4与VEGF的表达呈负相关。Kaplan Meier曲线显示 ,TGF β1、VEGF的阳性表达组与Smad4、TβRⅡ的?
OBJECTIVE:To evaluate the relationship between inactivation of TGF β 1 signaling pathway and pathogenesis of rectal cancer.METHODS:Twenty specimens of normal rectal adenoepithelium,thirteen specimens of rectal atypical hyperplasia and sixty specimens of different differentiated rectal cancers were tested by immunohistochemical techniques for expression of TGF β 1,TβRⅡ,Smad4 and VEGF.RESULTS:The expression of TGF β 1 was significantly higher in poorly differentiated adenocarcinoma (13/18),medium differentiated adenocarcinoma(17/23) and well differentiated adenocarcinoma (11/20) than that in group of atypical hyperplasia (21 5%), P <0 05,and had a significant relevance with regional node metastasis, P <0 01.The positive staining of TβRⅡ was higher in groups of normal rectal adenoepithelium(15/20) and atypical hyperplasia (7/13) than that was in poorly differentiated adenocarcinoma (3/18), medium differentiated adenocarcinoma (4/23) and well differentiated adenocarcinoma (4/20), P <0 05.The loss expressions of Smad4 in groups of of poorly and medium differentiated adenocarcinoma were observed in (13/18) and (16/23),respectively,and were significant higher than those were in groups of well differentiated adenocarcinoma (7/20),atypical hyperplasia (4/13) and normal rectal adenoepithelium (6/20), P <0 05.The expression of VEGF was significant higher in groups of poorly differentiated adenocarcinoma (12/18) and medium differentiated adenocarcinoma (16/23) than that was in well differentiated adenocarcinoma (5/20) and atypical hyperplasia (3/13), P <0 05.Expressions of TGF β 1 and VEGF were positively correlated,expressions of Smad4 and TβRⅡ were positively correlated and expressions of Smad4 and VEGF were negatively correlated.Kaplan Meier analyses revealed an impact on survival by TGF β 1,TβRⅡ,Smad4 and VEGF.CONCLUSIONS:The abnormal TGF β 1 signaling pathway and pathogenesis of rectal cancer are positively correlated.
出处
《肿瘤防治杂志》
2004年第1期73-77,共5页
China Journal of Cancer Prevention and Treatment
