摘要
目的 观察内源性血管形成抑制剂 (endostatin)对膀胱癌生长的抑制作用。方法 用大肠杆菌表达重组小鼠endostatin ,Ni NTA亲和层析纯化。予endostatin 2 0mg·kg-1 ·d-1 皮下注射T73 9膀胱癌荷瘤小鼠 ,观察肿瘤生长情况及对肿瘤血管形成的影响。结果 重组endostatin治疗后小鼠肿瘤生长缓慢 ,抑瘤率达 81 4%;肿瘤微血管密度 (MVD)明显低于对照组 (P <0 0 1)。结论 重组endostatm对膀胱癌的血管形成及生长有明显的抑制作用。
Objective To observe the inhibitory effect of endogenous angiogenesis inhibitor endostatin on the growth of bladder transitional cell carcinoma. Methods Recombinant mouse endostatin expressed by E. coli was purified with Ni NTA affinity chromatography and was administrated into mice bearing T739 bladder transitional cell carcinoma via subcutaneous injection at the dose of 20 mg·kg -1 ·d -1 . The tumor growth and angiogenesis were observed 10 days later. Results Tumors in mice treated with endostatin grew slowly and the inhibition rate reached 81 4%. Microvessel density(MVD) of tumor in treated group was significantly lower than that in the control group( P <0 01). Conclusion Recombinant mouse endostatin can markedly suppress bladder cancer growth and angiogenesis.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第22期1989-1991,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目 ( 3980 0 16 8)~~
