摘要
目的：为进一步探讨膀胱肿瘤的治疗途径。方法：利用Ｌｉｐｏｆｅｃｔａｍｉｎｅ将外源性野生型ｐ５３基因导入小鼠膀胱移行细胞癌ＢＳＴ７３９细胞中，并得到表达。结果：细胞在体外标准条件下，生长增殖率降低。流式细胞计检测：细胞周期分析显示Ｇ０＋Ｇ１细胞比例明显增高，凋亡指数升高，细胞生长速度减低，细胞增殖能力下降，体内接种致瘤性下降。结论：增加野生型ｐ５３的基固表达能抑制恶性肿瘤的生长。
Objective: The aim of this study is to present a new therapy for bladder cancer. Meth-ods: A mouse bladder transitional cell carcinoma cell line MBST739 was transfected with wt - p53 by Lipo-fectamine. The plasmid PC - SN3 with wt - p53 - cDN Aexpressed in the mouse bladder cancer cells. Re-sults: After wt - p53 transfection, the cell growth rate decreased in vitro under standard condition. FCMexamination disclosed that the cell ratio in GO + G1 and the apoptosis index increased. The number of Ag-NORs and the carcinogenicity of cells reduced- Conclusion: The results showed that the growth of cancercells could be suppressed by wt - p53 gene.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1999年第4期298-300,共3页
Chinese Journal of Clinical Oncology
基金
天津市自然科学基金
