摘要
目的 检测其组织细胞内DNA含量和端粒酶变化 ,探讨食管癌前病变的早期诊断方法。方法 电子胃镜下观察食管黏膜变化 ,用碘液染食管黏膜上皮 ,观察颜色变化 ,用流式细胞仪测定组织细胞的DNA增殖指数和端粒酶的表达水平。结果 正常组织和轻度不典型增生组织的DNA增殖指数 (DI)明显低于癌组织 (P <0 .0 5 ) ;正常组织端粒酶的含量明显低于癌组织 (P <0 .0 1)和中、重度不典型增生组织 (P <0 .0 5 ) ;轻度不典型增生组织端粒酶水平也低于癌组织和中、重度不典型增生组织 (P <0 .0 1)。结论 DNA指数、细胞增殖率的测定在癌前病变诊断方面的应用还有待进一步的研究 ;端粒酶表达在食管上皮中、重度不典型增生时就发生了明显变化 ,是食管病变的早期指标 。
Objective To discuss early diagnosis of precancerous lesions. Methods DNA content and telomerase activity were detected by flow cytometry.Results Differential index(DI) in normal tissue and atypical hyperplasia I was obviously inferior to that in carcinoma(P< 0.05); The telomerase activity of normal tissue was lower than that of cancer tissue(P< 0.01) and that of the atypical hyperplasia Ⅱ-Ⅲ tissue(P< 0.05); The telomerase activity in cancer and atypical hyperplasia Ⅱ-Ⅲ tissue was higher than that in atypical hyperplasia I(P< 0.05).Conclusion The determinations of DI were further studied to occurrence of esophageal carcinoma; The change of telomerase activity was sensitive to occurrence of esophageal carcinoma, and its application would be studied further in clinical practice.
出处
《临床荟萃》
CAS
北大核心
2004年第8期439-441,共3页
Clinical Focus
