摘要
目的 用肿瘤坏死因子α (TNF α)基因转导的人肝癌细胞作为疫苗接种小鼠 ,研究其对小鼠肝癌移植瘤的免疫排斥作用。方法 取昆明种小鼠 40只 ,分为 5组 ,每组 8只。分别采用经60 Co照射的TNF α基因转导的人肝癌细胞 (BEL 740 4 TNF Co组 )、未经60 Co照射的TNF α基因转导的人肝癌细胞 (BEL 740 4 TNF组 )、经60 Co照射的人肝癌细胞 (BEL 740 4 Co组 )以及未经60 Co照射的人肝癌细胞 (BEL 740 4组 )接种小鼠 ,生理盐水对照组仅注射生理盐水。然后将小鼠肝癌细胞株H2 2移植于上述 5组小鼠 ,观察肝癌移植瘤生长情况 ,并应用脱氧核糖核酸末端转移酶介导的缺口末端标记 (TUNEL)法观察肿瘤细胞凋亡指数。结果 BEL 740 4 TNF Co组小鼠接种经60 Co照射的TNF α基因转导的人肝癌细胞疫苗后 ,肝癌移植瘤成瘤率下降 ,瘤体生长受抑制 ,细胞凋亡指数增高 ,与BEL 740 4 Co组、BEL 740 4组及生理盐水对照组相比 ,差异有显著性 (P <0 .0 1) ,但与BEL 740 4 TNF组相比 ,差异则无统计学意义 (P>0 .0 5 )。结论 TNF α基因转导的人肝癌细胞疫苗对小鼠肝癌移植瘤有较强的免疫排斥作用。
Objective Using human tumor necrosis factor-alpha (TNF-α) gene-transduced human liver cancer cell BEL-7404 as tumor vaccine, to study the effect of immune rejection to mice liver cancer implanted tumors. Methods Mice were divided into five groups, and were inoculated with TNF-α gene-transduced BEL-7404 cells which irradiated with 60 Co (BEL-7404-TNF-Co group), TNF-α gene-transduced BEL-7404 cells (BEL-7404-TNF group), BEL-7404 cells (BEL-7404 group), BEL-7404 cell irradiated with 60 Co (BEL-7404-Co group) respectively. Normal saline was injected in control group. Then mice liver cancer H22 cells were implanted to each group, the growth of mice liver cancer implanted tumors was observed. The apoptosis index of implanted tumors was detected by TUNEL method.Results Compared to BEL-7404 group,BEL-7404-Co group and control group, the tumor vaccine which did not transduce with TNF-α gene and the control group, the tumorigenesis rate of liver cancer implanted tumors was reduced, the growth of implanted tumors was inhibited and the apoptosis of implanted tumors was increased in BEL-7404-TNF-Co group, P <0.01.There was no difference between BEL-7404-TNF-Co group and BEL-7404-TNF group, P >0.05. Conclusion Human tumor necrosis factor-alpha gene-transduced human liver cancer cell can be used as tumor vaccine, it has quite strong effect of immune rejection to mice liver cancer implanted tumors.
出处
《中国普外基础与临床杂志》
CAS
2003年第4期366-369,共4页
Chinese Journal of Bases and Clinics In General Surgery
基金
广西壮族自治区教育厅重点资助科研项目 (桂教1 9982 8)~~
