摘要
目的 :用肿瘤坏死因子基因转导的人肝癌细胞作为肿瘤疫苗 ,研究其对小鼠机体免疫活性的影响。方法 :小鼠分 5组 ,采用经 6 0 Co照射的含人肿瘤坏死因子基因的人肝癌细胞 (BEL- 74 0 4 - TNF- 6 0 Co)、未经 6 0 Co照射的含人肿瘤坏死因子基因的人肝癌细胞 (BEL- 74 0 4 - TNF)、经 6 0 Co照射的人肝癌细胞 (BEL- 74 0 4 - 6 0 Co)、未经 6 0 Co照射的人肝癌细胞 (BEL- 74 0 4 )和生理盐水分别接种小鼠 ,用 MTT法检测各组小鼠脾脏 NK细胞活性 ,用 SABC免疫组化法检测 T淋巴细胞亚群。结果 :肿瘤疫苗接种小鼠后 ,BEL- 74 0 4 - TNF- 6 0 Co组小鼠脾脏 NK细胞活性及 T淋巴细胞亚群 CD+ 4、CD+ 8增高 ,与 BEL- 74 0 4组、BEL- 74 0 4 - 6 0 Co组、生理盐水组相比差异有显著意义 (P <0 .0 5、P<0 .0 1)。BEL- 74 0 4 - TNF- 6 0 Co组和 BEL- 74 0 4 - TNF组相比 ,NK细胞活性、T淋巴细胞亚群 CD+ 4、CD+ 8均无显著差异 (P >0 .0 5 )。结论
Objective:Using human tumor necrosis factor alpha (TNF α) gene transduced human hepatic carcinoma cell BEL 7404 as a tumor vaccine, to study its effect on systemic immunity of mice Methods:Mice were divided into five groups, and were inoculated with TNF α gene transduced BEL 7404 (BEL 7404 TNF), TNF α gene transduced BEL 7404 which irradiated with 60 Co (BEL 7404 TNF 60 Co), BEL 7404 , BEL 7404 irradiated with 60 Co(BEL 7404 60 Co), respectively Normal Saline was injected in control group The natural killer cell activity were assayed by MTT method, and the contents of CD + 3,CD + 4,CD + 8 of T lymphocyte subpopulation of the mice was detected by SABC immunohistochemical method Results:After being inoculated with the tumor vaccine, BEL 7404 TNF 60 Co group was compared to BEL 7404 group,BEL 7404 60 Co group and NS group The natural killer cell activity and the population of CD + 4,CD + 8 of the mice increased significantly(P<0 05 ,P<0 01) There was not significant difference between BEL 7404 TNF 60 Co group and BEL 7404 TNF group in NK activity, and CD + 4 and CD + 8 subpopulation(P>0 05) Conclusions:Human tumor necrosis factor alpha gene transduced human hepatic carcinoma cell BEL 7404 TNF 60 Co can be used as a tumor vaccine to improve the systemic immunity of mice
出处
《广西医科大学学报》
CAS
2003年第1期58-61,共4页
Journal of Guangxi Medical University
关键词
肿瘤坏死因子
基因转导
肿瘤疫苗
小鼠
机体免疫
tumor necrosis factor alpha
gene transduced tumor vaccine
hepatic carcinoma
immunity
