摘要
目的:观察随着脊髓不完全损伤后大鼠后肢功能恢复,内源性神经营养因子表达的变化。方法:实验于1998-04/09在解放军第三军医大学野战外科研究所完成。Wistar大白鼠44只,脊髓功能观测组12只;脊髓形态观察组32只,随机分为正常组2只、手术假伤组15只和脊髓腹侧损伤组15只。脊髓功能观测组12只和脊髓腹侧损伤组15只造脊髓损伤模型;手术假伤组15只进行造模处理,但不损伤脊髓。脊髓功能观测组于脊髓受压前及脊髓受压迫损伤后6,24,72h,7,14,21d对受试动物进行后肢行为功能评定。脊髓形态观察组32只实验动物进行免疫组织化学染色,随机计数50个细胞,观察神经营养素脑源性神经营养因子、胶质细胞源性神经营养因子、神经生长因子、神经营养素3的表达。结果:44只实验大鼠均进入结果分析。①脊髓致伤后受试动物后肢出现明显瘫痪,随着时间的延长,脊髓功能得到逐步恢复。其中以伤后3～14d脊髓功能恢复最快,以后恢复较缓慢。②自伤后3h开始,脑源性神经营养因子、胶质细胞源性神经营养因子、神经生长因子、神经营养素3的表达开始增加,并于伤后72h达到高峰;1周内神经营养素维持在相对较高的表达水平,2周后这几种神经营养素的表达明显减弱[伤后3h:(14.82±4.93),(9.77±4.97),(2.27±1.85),(10.35±5.56);伤后72h:(45.22±15.61),(34.54±12.56),(13.89±7.58),(33.2±11.53);伤后1周:(31.94±12.82),(15.69±11.53),(7.17±4.92),(16.74±13.2);伤后2周:(14.02±7.36),(6.97±4.05),(2.27±1.87),(9.7±5.62)]。结论:伤后脊髓组织内源性神经营养因子的过度表达参与了伤后2周内脊髓功能的快速恢复,但内源性神经营养因子的表达是短暂的,延续内源性神经营养因子的表达有望进一步促进脊髓功能恢复。
AIM： To observe the functional recovery of hindlimb and changes of the expression of endogenous neurotrophic factors in rats after incomplete spinal cord injury.
METHODS： The experiments were done in the Research Institute of Field Surgery, the Third Military Medical University of Chinese PLA from April to September in 1998. Forty-four Wistar rats were divided into spinal cord function observation group （n=12） and spinal cord morphologic observation group （32 rats）, and the latter group was then randomly divided into normal group （n=2）, sham-operated group （n=15） and incomplete spinal cord injury group （n=15）. Rats in spinal cord function observation group （n=12） and incomplete spinal cord injury group （n=15） were made into models of spinal cord injury, the same operations were made in the sham-operated group （n=15）, but the spinal eord was not injured. In the spinal cord function observation group, hindlimb hehavior function was evaluated before compression and at 6, 24 and 72 hours and 7, 14 and 21 days after compression respectively. In the spinal cord morphologic observation group （n=32）, immunohistochemical staining was done, 50 cells were randomly counted to detect the expressions of brain-derived neurotrophie factor （BDNF）, glial cell-line derived neurotrophic factor （GNDF）, nerve growth factor （NGF） and neurotrohin-3 （NT-3）.
RESULTS： All the 44 rats were involved in the analysis of results. ① After spinal cord injury, the ohvious paralysis of hindlimb occurred in the rats, and the spinal cord function gradually recovered with time prolonged, it recovered rapidly at 3-14 days after injury and then the slowed down. ② From 3 hours after injury, the expressions of BDNF. GNDF, NGF and NT-3 hegan to increase, and reached the peak value at 72 hours, the expressions kept at a higher level within 1 week, and obviously reduced after 2 weeks [at 3 hours： （14.82±4.93）, （9.77±4.97）, （2.27 ±1.85）, （10,35 ±5.56）; at 72 hours （45.22±15.61）, （34.54±12.56）, （13.89±7,58）, （33,2±11.53）; at I week： （31.94_±12,82）, （15.69±11.53）. （7.17±4.92）, （16.74±13.2）： （14.02±7.36）. （6.97±4.05）, （2.27±1.87）, 0.7±5.62）].
CONCLUSION： The overexpressions of the emtogenous neurotrophins in spinal cord after injury are inwdved in the rapid recovery of spinal cord function within 2 weeks after injury, hut the expressions of endogenous neurotrophins are transient, it is prospective that prolonging the expression of neurotrophins may further improve the functional recovery of spinal cord.
出处
《中国临床康复》
CSCD
北大核心
2006年第4期104-106,F0003,共4页
Chinese Journal of Clinical Rehabilitation
