摘要
目的 探讨小鼠光气染毒剂量与肺水肿形成的关系及其对肝脏的过氧化损伤。方法 5 0只小鼠 ,雌雄各半 ,随机分为 5组。正常对照组小鼠放置染毒柜中 5min ,染毒组小鼠分别给予 3 2、3 9、46、5 3mg L的光气 (各 10只 ) ,染毒时间为 5min。染毒后 4h ,测定各组小鼠肺脏的湿干比、肝脏的丙二醛 (MDA)含量、总超氧化物歧化酶 (T SOD)活力及进行肝脏HE染色。结果 随着光气染毒剂量的增加 ,小鼠肺脏的湿干比增加 ;当染毒剂量为 3 2mg L ,肝脏的MDA含量[(2 10 3 1± 44 61) μmol g]和T SOD活力 [(3 2 5 1± 6 10 )U g]比正常组 [(15 7 2 1± 18 17) μmol g ,(2 2 3 8± 6 0 2 )U g]升高 (P <0 0 5 ) ;光镜下 ,光气染毒的肝组织肝细胞呈现空泡样变性。结论 光气染毒可造成小鼠肺水肿 ,引起肝脏过氧化损伤。
Objective To study the relation between phosgene dose and pulmonary edema formation and peroxide injury in liver in mice.Methods 50 mice were divided into 5 groups:negative group,32 mg/L group,39 mg/L group,46 mg/L group,53 mg/L group.mice were poisoned by phosgene with different doses for 5 minutes.After 4 hours,all mice were saccrificed and lung wet/dry weight ratio,content of malondialdehyde (MAD) in serum and liver,activities of the total superoxide dismutase (T\|SOD) in liver were determined and liver tissue was stained.Results With phosgene dose increasing,lung wet/dry weight ratio increased.32 mg/L group posioned by phosgene,MDA content [(210.31±44.61) μmol/g] and T\|SOD activity [(32.51±6.10) U/g] in liver significantly increased ( P<0 05 ) in mice, compared with negative group [(157.21±18.17) μmol/g,(22.38±6.02) U/g].Vacuolar degeneration was found in hepatocyte in poisoning mice.Conclution\ phosgene can induce mice to form pulmonary edema and peroxidant damage in liver.
出处
《卫生毒理学杂志》
CSCD
北大核心
2004年第1期10-12,共3页
Journal of Health Toxicology
基金
军队"十五"指令课题 (0 1L0 77)
