摘要
By using ion-exchange preparative chromatography (IEPC) and reversed-phase high performance liquid preparative chromatography(RP-HPLPC), thymosin α 1 was isolated and purified from the crude product synthesized by the solid-phase peptide synthesis(SPPS) method. The purity of the final product reached 95% through ion-exchange chromatography on DEAE Sepharose Fast Flow chromatography and Delta-Pak TM C18 purification after optimizing the chromatographic conditions. The capacity of purification process was 50mg/circle. The total yield was 36%. The technology is simple and reliable, and can be scaled up easily.
By using ion-exchange preparative chromatography (IEPC) and reversed-phase high performance liquid preparative chromatography(RP-HPLPC), thymosin α 1 was isolated and purified from the crude product synthesized by the solid-phase peptide synthesis(SPPS) method. The purity of the final product reached 95% through ion-exchange chromatography on DEAE Sepharose Fast Flow chromatography and Delta-Pak TM C18 purification after optimizing the chromatographic conditions. The capacity of purification process w...
基金
天津市自然科学基金资助项目 (No 993 80 43 11)
关键词
胸腺素Α1
液相色谱法
分离
纯化
固相化学合成
thymosin α1
solid-phase peptide synthesis（SPPS）
ion-exchange chromatography（IEC）
reversed-phase high performance liquid chromato-graphy （RP-HPLC）
isolation
purification
