摘要
目的 :探讨周期性牵张力对人牙周膜细胞MMP -3及TIMP -1蛋白表达的影响 ,进一步阐明正畸牙牙周组织改建过程中ECM代谢调节的分子机制 .方法 :通过体外细胞培养加载系统 ,选择频率为 6周 /min ,弹性基底膜发生 12 %形变率的周期性牵张力 ,利用免疫组化及夹心ELISA检测技术 ,观察HPDLC表达MMP -3及TIMP -1的相对强度。结果 :HPDLC在体外表达MMP -3及TIMP -1。加载 3d后 ,MMP -3表达明显增强 ,与对照组相比具有显著性差异。而TIMP -1对机械力无应答。结论 :在一定条件下 ,周期性牵张力可显著影响HPDLCMMP -3蛋白的表达。为机械力作用下MMP
Objective:To investigate the effects of the mechanical stretching on the expression of MMP-3 and TIMP-1 in human periodontal ligament cells (HPDLC), and to unveil the molecular mechanism of ECM metabolism during orthodontic periodontal tissue remodeling.Method: HPDLC were subjected to 12% elongation by strain unit at 6cycles/min (i.e. 5-s elongation and 5-s relaxation) for 1, 3and 5days in this experiment. Expression and secretion of MMP-3 and TIMP-1 were determined by immunohistochemistry and sandwich-in ELISA.Result: The expression and secretion of MMP-3 were significantly induced by loading cyclic stretch for more than 3 days.However, stretch had no effect on the expression and secretion of TIMP-1.Conclusion: The results suggest that tension-force may affect ECM metabolism depending on the funcuional role of MMP-3.
出处
《临床口腔医学杂志》
2004年第4期195-198,共4页
Journal of Clinical Stomatology
基金
国家自然科学基金资助项目 (30 30 0 393)
关键词
细胞外基质
基质金属蛋白酶-3
金属蛋白酶组织抑制因子-1
牙周膜细胞
机械张力
extracellular matrix
matrix metalloproteinase-3 (MMP-3)
tissue inhibitor of metalloproteinase-1(TIMP-1)
periodontal ligament cells
mechanical stress
