摘要
目的:研究周期性牵张力对人成骨细胞基因表达谱的影响,为进一步研究机械应力状态下骨改建机理提供思路和线索。方法:通过体外细胞加载系统对培养的人成骨细胞施加8%形变率、6周/min的周期性牵张力24h,应用基因表达谱芯片技术对人成骨细胞中的mRNA表达水平进行对比杂交分析,检测周期性牵张力加载组成骨细胞基因表达谱的变化。结果:在所分析的21073条人类功能基因中,加载组与未加载组人成骨细胞之间差异表达显著的基因有2498条,其中表达上调的有899条(2.0倍以上),表达下降的有1599条(0.5倍以下)。这些基因表达产物涉及细胞信号转导、细胞周期调节等多个方面。结论:周期性牵张力对成骨细胞多种基因的表达产生了影响,成骨细胞的应力反应是一个复杂的,多基因参与调控的过程。这些变化的基因可能与成骨细胞的机械应力反应有关。
Objective To study effects of cyclic tensile strain on gene expression profiles in human osteoblasts in vitro.Methods Human osteoblasts were cultured on flexible-bottomed plates and subjected to 8% elongation by strain unit at 6 cycles/min（i.e.5-s elongation and 5-s relaxation）for 24 hours in the experimental groups.Control group were not exerted any strain.Both of them were examined by gene expression profiles chip technology.Results A series of differentially expressed genes were found between experimental group and control group.899 up-regulated and 1599 down-regulated genes were identified among the 2 498 differentially expressed genes.Conclusion The results shows that the response of human osteoblasts to cyclic tensile strain in gene level was complicate.These differentially expressed genes are necessary genes related to osteoblasts response to mechanical strain.
出处
《中国美容医学》
CAS
2007年第10期1410-1413,共4页
Chinese Journal of Aesthetic Medicine
基金
国家自然科学基金资助项目(30570455)
