摘要
目的 了解小鼠全脑X射线照射后脑组织急性损伤的表达和中药的保护作用。方法 将 112只BALB/C雄性小鼠分为对照组、单纯照射组、照射加地塞米松组、照射加山莨菪碱组、照射加复方丹参组。全脑照射 2 5Gy后 ,测定各组脑组织和血浆中脂质过氧化物 (LPO)含量的改变及脑组织中血管内皮细胞细胞间黏附分子 (ICAM 1)的表达情况。用方差分析方法检测各实验组脑组织和血浆中LPO含量差别 ,用 χ2 检验分析ICAM 1组间表达差异。结果 (1)BABL/C小鼠全脑照射后 10h ,脑组织匀浆中LPO的含量明显高于未照射组 (P <0 .0 1) ,复方丹参可显著降低照射引起的LPO升高(P <0 .0 5 ) ,血浆中LPO的含量各组无显著差异。 (2 )各组ICAM 1的表达有时间规律 ,照射后 2 4hI CAM 1表达组间存在一定差别 ,与单纯照射组相比 ,照射加复方丹参组及地塞米松组可明显抑制I CAM 1的表达 (P <0 .0 1)。结论 脂质过氧化物含量的改变和ICAM 1表达的增强是放射诱导的脑组织急性损伤的观察指标 ,中药复方丹参对放射诱发的脑组织急性损伤有保护作用。
Objective To understand the expression of acute brain injury induced by radiation and the protective effect of traditional Chinese Medicine in BALB/C mouse. Methods The whole brain of BALB/C mouse was irradiated to a dose of 25?Gy using a 6 MV X linear accelerator. Ten hours later, the brain tissue and blood sample were taken. Thiobarbituric acid reaction was used to detect the malonaldehyde substitute for the lipid peroxide. Immunohistochemical method was used to detect the expression of ICAM-1 on D1, 2, 3, and 10 after having received radiation. One-Way ANOVA was used to evaluate the differences in the values of LPO in the brain tissue and plasma between the groups. The difference of expression of ICAM-1 between the groups was compared by χ2 method. Results Two hundred and twelve female BALB/C mice were divided into five groups: Control group, Radiation alone group (R), R+dexamethasone group, R+654-2 group and R+Salvia Miltiorrhiza group. The contents of LPO in the mouse brain tissue 10 hours after 25?Gy of whole brain irradiation were as follows (mean±standard error): Control group (1975.5±94.2)nmol/g, Radiation alone group (R)(3417.3±109.7)nmol/g, R+dexamethasone group(3113.6±178.1)nmol/g, R+654-2 group (3406.4±159.1)nmol/g, R+Salvia Miltiorrhiza group(2981.5±140.1)nmol/g. Salvia Miltiorrhiza significantly reduced the LPO increase induced by irradiation (P<0.05). There were no significant differences between the other groups in the change of LPO in the plasma 10 hours after whole brain irradiation. The expression of ICAM-1 after whole brain irradiation was time-dependent. There was an increase of expression of ICAM-1 24 hours after irradiation, reaching the peak at 48 hours. Salvia Miltiorrhiza and dexamethasone strongly inhibited the expression of ICAM-1 when compared with radiation only, with the difference significant (P<0.01). Conclusions The change of LPO content in the BALB/C mouse brain tissue and the increase in expression of ICAM-1 on the brain vascular endothelial cell can act as indexes of acute brain injury induced by radiation. Traditional Chinese medicine Salvia Miltiorrhiza has a protective effect on radiation-induced acute brain injury.
出处
《中华放射肿瘤学杂志》
CSCD
北大核心
2003年第2期112-115,共4页
Chinese Journal of Radiation Oncology
基金
国家自然科学基金资助项目 ( 3 9470 2 2 6)
