摘要
目的探讨脉络膜黑色素瘤中微血管密度(MVD)与临床病理学预后指标及巩膜导管受侵与否的关系。方法应用高碘酸Schiff(PAS)特殊染色法显示血管基底膜,免疫组化染色法检测65例脉络膜黑色素瘤中血管内皮细胞特异性标记物第八因子相关抗原(FⅧ-RAg)的表达,并通过形态学计量法计数肿瘤内微血管密度。结果肿瘤微血管密度与脉络膜黑色素瘤患者年龄、肿瘤位置、肿瘤高度无关,与肿瘤最大基底直径(LTD)、肿瘤大小分组、细胞类型、侵犯巩膜导管与否有关。结论肿瘤血管增生与临床病理学预后指标之间有密切关系,提示MVD可能成为预测脉络膜黑色素瘤预后的重要指标之一。
Objective To investigate t he relationship between microvessel density(MVD)and clinicopathologic prognost ic predictors in choroidal melanoma.Methods Sixty-five cases of choroidal mel anoma were selected.MVD was identified by periodic acid Schiff(PAS)staining of periendothelial basement membrane without hematoxylin counterstaining and b y immunohistochemical staining of vascular endothelial cells with factorⅧ-rela ted antigen(FⅧ-RAg).Tissure sections stained by PAS were examined with a gr een narrow band pass filter at540nm and produced a magenta color in tumor vas cular.Results There was no correlation between the MVD and tumor location,as w ell as the tumor height and the patient ’s age(P>0.05).However,signif icant correlation was found between the MVD and the largest tumor diameter(LTD ),as well as tumor size,cell type and tumor invasion to the scleral emissaria (P<0.01).Conclusion Angiogenesis is tightly correlated with clinicopatho logic prognostic factors,such as LTD,tumor size,cell type and invasion.MVD m ay be a useful prognostic indicator in choroidal melanoma.
出处
《眼科研究》
CSCD
北大核心
2004年第1期17-20,共4页
Chinese Ophthalmic Research
