摘要
采用钙荧光指示剂Fura—2/Am定量测试法检测了静息淋巴细胞胞浆内游离钙浓度,并以Ca^(2+)载体A23187作为阳性对照.观察了(一)蝙蝠葛苏林碱(8701)对兔淋巴细胞胞浆内Ca^(2+)浓度的影响。结果表明:8701的各种实验浓度均可显著地促进淋巴细胞内Ca^(2+)浓度增加,在浓度为10^(-4)mol·L^(-1)时,增加细胞内Ca^(2+)浓度的作用显著高于Ca^(2+)载体A23187的作用(P<0.01),提示该药有可能作为淋巴细胞的刺激剂或在高浓度时可能造成淋巴细胞的Ca^(2+)中毒作用。
This paper reported the estimation of free calcium concentration in rabbit resting lymphocytes, and the effect of (-) Daurisoline (8701) on the cyto-plasmic free calcium concentration by using fluorescent indicator Fura -2/Am estimating technique. Results were compared with the effect of the Ca2-ionophore A23187.
The results indicated that the free calcium concentration of resting lymphocytes was 109. 6nmol · L-1, after 10-5 mol· L-1,10-4.5 mol · L-1,and 10-4 mol · L-1
-)Daurisoline administration, the cyto-plasmic free calcium concentration increased to 178. 8%, 204. 4%and 817. 5% respectively, while same concentration A23187 only led the increase of 164. 6% , 198.4% and 327% respectively.
Results suggested that ( - )Daurisoline may be a Ca2- agonist and may induce the cytotoxicity effect on lymphocytes at high concentr ation.
出处
《中国药理学通报》
CAS
CSCD
北大核心
1992年第3期198-201,共4页
Chinese Pharmacological Bulletin
关键词
蝙蝠葛苏林碱
淋巴细胞
钙
(-)daurisoline
lymphocytes
calcium
fluorescent indicator
fu-ra -2/Am
