摘要
目的:探讨联合分析脑脊液中Tau蛋白、β淀粉样蛋白(Aβ)1-40、Aβ1-42(43)含量作为生化指标对诊断阿尔茨海默病(Alzheimer'sdis-ease,AD)的意义。方法:采用夹心酶联免疫(sELISA)法测定上述生化指标,分析了21例AD,28例非AD痴呆,35例其他神经系统疾病患者,50例正常对照者脑脊液中Tau、Aβ1-40、Aβ1-42(43)水平的差异。结果:Tau水平随年龄而增加,AD组Tau水平为(491.5±35.7)ng/L且与临床进程存在相关性,Aβ1-42(43)水平为(109.9±73.2)pmol/L,Aβ1-40/Aβ1-42(43)为(16.03±4.07)。AD组Tau水平、Aβ1-40/Aβ1-42(43)比率显著高于其他组(P<0.001),Aβ1-42(43)水平低于其他组。结论:同时测定脑脊液中的Tau、Aβ1-40、Aβ1-42(43)的含量对于AD的诊断有良好的特异性,对于AD与其他类型的痴呆和其他神经系统疾病的鉴别诊断具有意义。
AIM:To investigate the diagnostic accuracy of concentrations of Tau protein,amyloid βprotein(Aβ) 1-40,Aβ1-42(43) in cerebrospinal fluid(CSF) among patients with Alzheimer's disease(AD). METHODS:Sandwich Enzyme linked immunosorbent assay(sELISA) was used for the measurement of the levels of Tau,Aβ1-40 and Aβ1-42(43) among 21 patients with AD,28 with other dementias,35 with other neurological diseases,and 50 normal control subjects. RESULTS:The CSF concentration of Tau protein increased with age.Among AD patients,the concentration of Tau protein was(491.5±35.7) ng/L and it was correlated positively with the severity of AD. The concentrations of A β1-40 and Aβ1-42(43) were(109.9±73.2) and(16.03±4.07) pmol/L respectively.The concentration of Tau protein and the ratio of Aβ1-40/Aβ1-42(43) were significantly higher in AD group than in all other groups. CONCLUSION: Combined interpretation of CSF concentration of Tau protein, Aβ1-40 and Aβ1-42(43) has high diagnostic specificity for AD and can be used to differentiate AD from other dementias and neurological diseases.
出处
《中国临床康复》
CSCD
2004年第10期1982-1983,共2页
Chinese Journal of Clinical Rehabilitation
