摘要
目的 观察Rb反义寡核苷酸 (AS -ODN)对人成骨肉瘤 (MG - 63)细胞凋亡及化疗药物敏感性的影响。方法 人工合成RbAS -ODN、S -ODN(正义寡核苷酸 )经脂质体 (Lip)包裹转染MG - 63细胞。采用WesternBlot检测转染前后Rb蛋白表达状况 ,流式细胞术测定MG - 63细胞转染前后阿霉素 (Adriamycin ,ADM)诱导细胞凋亡程度 ,MTT法观察化疗药敏的变化。结果 经RbAS -ODN处理的MG - 63细胞Rb蛋白表达量明显下降 ,RbAS -ODN处理的MG - 63细胞加ADM作用后与对照组比较 ,细胞凋亡率明显下降 ,化疗药物敏感性降低。结论 RbAS -ODN阳离子脂质体转染具有抑制化疗药诱导骨肉瘤MG - 63细胞凋亡的作用及降低化疗药物敏感性作用。
Aim To investigate the effects of Rb AS-ODN on apoptosis and drug sensitivity of osteosarcoma cell. Methods MG-63 cells were transfected with Rb AS-ODN by cationic liposome, S-ODN and LP in vitro.The expression of pRb treated with or without AS-ODN ,S-ODNand LP in MG-63 cells was observed by Western blot. The Flow cytometer(FCM) was used to examine apoptosis induced by ADM in MG-63 cells. The MTT assay was used to measure chemotherapeutic agent-sensitivity of MG-63 cells treated with ADM. Results The level of pRb expression in the MG-63 osteosarcoma line transfected with Rb AS-ODN obviously reduced compared with that of controls ,which were treated with S-ODN,LP and non-transfected(P<0.05). The percentage of the apoptotic cells and drug-sensitivity of MG-63 cells treated with Rb AS-ODN and ADM decreased compared with that of the controls(P<0.05). Conclusion The transfection of Rb AS-ODN delivered by cationic liposome could inhibit apoptosis induced with ADM in MG-63 cells and decrease drug-sensitivity of MG-63 cells to ADM.
出处
《南华大学学报（医学版）》
2004年第1期54-57,共4页
Journal of Nanhua University(Medical Edition)
关键词
骨肉瘤
寡聚核苷酸
RB基因
凋亡
药物敏感性
osteogeinc sarcoma
oligonucleotides
Rb gene
apoptosis
chemosensitivity
