摘要
目的 探讨丙型肝炎病毒核心基因 (hepatitisCviruscoregene ,HCVC基因 )调控端粒酶逆转录酶(humantelomerasereversetranscriptase,hTERT)基因表达在肝门部胆管癌发生中的作用。方法 将构建成功的HCVC基重组真核表达载体pcDNAHCV -C和空载体与绿色荧光蛋白基因共转染人胆管正常上皮细胞 (hu mannormalbiliaryepithelialcells ,BEC)和胆管癌细胞QBC939,RT -PCR和免疫组织化学方法分析转染后细胞内hTERTmRNA和蛋白的表达。结果 pcDNAHCV -C在BEC和QBC939细胞中的转染率为 16 %和 32 % ,转染HCVC基因的BEC组表达hTERTmRNA ,而转染空载体的BEC不表达hTERTmRNA ;表达HCVC蛋白QBC939中hTERTmRNA和蛋白的表达明显高于空载体组。结论 转染的HCVC基因可以上调hTERT基因的表达 。
Objective To study the effect of human telomerase reverse transcriptase (hTERT)mRNA gene expression controlled by hepatitis C virus core gene(HCV C gene)on occurrenc of human biliary carcinoma.Methods The eukaryotic expression vector recombination with HCV C gene(pcDNA3-HCVC)and the vector-alone were co-transfected with enhanced green fluorescent protein(EGFP)into biliary carcinoma cell(QBC939)and human normal biliary epithelial cells(HBEC).The reverse transcription PCR(PT-PCR)and immunohistochemical methed were used to analyze the expression of hTERT mRNA and protein.Results The transfection efficiency of pcDNAHCV-C,were about 16%and 32% in QBC939 and HBEC.The HBEC transfected by HCV C gene express hTERT mRNA and there was no expression of hTERT mRNA in HBECs when transfected blank vector,but a dramatic phenomenon was observed that the expression of hTERT mRNA and protein assayed in QBC939 was more strikingly increased when transfected with HCV C expression vector than that transfected with blank vector.Conclusion HCV C gene transfection can up-regulate the expression of hTERT mRNA in biliary cancinoma cells,it maybe a way the HCV gene evoke biliary carcinoma.
出处
《现代肿瘤医学》
CAS
2004年第2期86-89,共4页
Journal of Modern Oncology
基金
中国博士后科学基金资助项目 ( 2 0 0 2 0 3 12 91)
