摘要
目的 探讨反义寡核苷酸 (ODNs)对丙型肝炎病毒 (HCV)转染胆管癌细胞中HCV的抑制作用。方法 通过半定量逆转录 聚合酶链反应 (RT PCR)检测HCVmRNA表达的变化 ,观察不同浓度 (0～ 6 .0 μmol/L)的HCV核心区ODNs对体外丙肝病毒感染胆管癌细胞 (QBC939)的抑制作用 ;同时以裸鼠为研究对象进一步观察ODNs对HCV的体内抑制作用。结果 ODNs可有效地进入靶细胞在体外与HCV核心基因杂交结合 ,使HCVmRNA的表达明显降低 ,终浓度为 6μmol/L的ODNs作用下无HCVmRNA表达。动物实验观察ODNs组在裸鼠中的瘤体平均直径小于对照组、PBK HCVC转染组 (P <0 .0 1 ) ,成瘤率 2 5 % (5/ 2 0 )低于PBK HCVC转染组 65 % (1 3/2 0 ) (P <0 .0 5) ,成瘤时间 1 5 .3d较对照组 1 2 .0d、PBK HCVC转染组 1 0 .1d延迟 (P <0 .0 1 )。
Objective To study the inhibitory effect of antisense oligodeoxynucleotides (ODNs) on hepatitis C virus (HCV) in cholangiocarcinoma.Methods The QBC939 cells transfected by PBK HCVC, a recombinant HCV containing HCV core gene, were treated by 14 mers ODNs (0 6.0?μmol/L) complementary to HCV core genomic region. With a half ration, the variation of level of HCV mRNA was detected by RT PCR. Moreover, the inhibitory effect of ODNs was observed in nude mice.Results The 14 mers complementary ODNs showed effective inhibition on HCVmRNA and unexpression of HCVmRNA at 6?μmol/L. The mean tumor size of PBK HCVC transfected group and control group were greater than that of ODNs group (P<0.01). Tumors were found at 65% (13/20) and 25% (5/20) among PBK HCVC transfected and ODNs group (P<0.05) respectively. The time of tumor appearance in PBK HCVC transfected group (10.1 days) and control group (12.0 days) were longer than that of ODNs group (15.3 days) (P<0.01).Conclusion A potential therapeutics for ODNs in the treatment of HCV infected cholangiocarcinoma was revealed.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2002年第5期398-400,共3页
Chinese Journal of Experimental Surgery
