摘要
AIM:To explore the expression of heparanase gene, CD44v6,MMP-7 and nm23 proteins in human gastric carcinoma as well as their relationship with the dinicopathological factors.METHODS: Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the expressions of heparanase mRNA in 43 human gastric carcinomas and 10 adjacent normal gastric tissues. Streptavidin-peroxidase (S-P) two step method was used to measure the immunohistochemical expression of CD44v6,MMP-7 and nm23protein in 43 human gastric carcinomas.RESULTS:The expression of heparanase gene was positivein 29 cases of gastric cancer with a positive rate of 67.4%,which was significantly higher than those in adjacent normal gastric tissues (P<0.05).The heparanase mRNA expression was significantly related to advanced stage of disease, serosal infitration,lymph node metastasis and size of tumors (P<0.05),but not related to tumor location, gross and histological types of the cancer, peritoneal dissemination and liver metastasis(P>0.05).The positive rate of CD44v6,nm23 and MMP-7 proteins was 76.7%,37.2% and 60.5%,respectively.The CD44v6 protein expression was significantly related to serosal infiltration,lymph node metastasis and TNM stage of disease(P<0.05).The nm23 protein expression was significantly related to the tumor gross appearance,lymph node and peritoneal metastasis (P<0.05).The MMP-7 protein expression was significantly related to serosal infiltration and TNM stage of disease (P<0.05).In an attempt to measure the association between these agents, we found that the expression of heparanase mRNA had significantly negative correlation with the expression of CD44v6 and MMP-7 protein (P<0.05), the expression of MMP-7 protein had significantly positive correlation with the expression of CD44v6 protein (r=0.568, P<0.05), the expression of MMP-7 protein had no correlation with the expression of nm23 protein (P>0.05),and the expression of nm23 protein had no correlation with the expression of CD44v6 protein (P>0.05).CONCLUSION: Despite their different mechanisms of action, heparanase, CD44v6 and nm23 may play important roles in the invasive infiltration and lymph node metastasis in gastric carcinomas. Instead of metastatic spreading, MMP-7 may be just related to the invasion of gastric carcinomas.However, co-detection of these factors may be important to predict metastatic spreading in such patients.
AIM:To explore the expression of heparanase gene,CD44v6, MMP-7 and nm23 proteins in human gastric carcinoma as well as their relationship with the clinicopathological factors. METHODS:Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the expressions of heparanase mRNA in 43 human gastric carcinomas and 10 adjacent normal gastric tissues.Streptavidin-peroxidase (S-P) two step method was used to measure the immunohistochemical expression of CD44v6,MMP-7 and nm23 protein in 43 human gastric carcinomas. RESULTS:The expression of heparanase gene was positive in 29 cases of gastric cancer with a positive rate of 67.4%, which was significantly higher than those in adjacent normal gastric tissues (P<0.05).The heparanase mRNA expression was significantly related to advanced stage of disease,serosal infiltration,lymph node metastasis and size of tumors (P<0.05), but not related to tumor location,gross and histological types of the cancer,peritoneal dissemination and liver metastasis (P>0.05).The positive rate of CD44v6,nm23and MMP-7 proteins was 76.7%,37.2% and 60.5%,respectively.The CD44v6 protein expression was significantly related to serosal infiltration,lymph node metastasis and TNM stage of disease (P<0.05).The nm23 protein expression was significantly related to the tumor gross appearance,lymph node and peritoneal metastasis (P<0.05).The MMP-7 protein expression was significantly related to serosal infiltration and TNM stage of disease (P<0.05).In an attempt to measure the association between these agents,we found that the expression of heparanase mRNA had significantly negative correlation with the expression of CD44v6 and MMP-7 protein (P<0.05),the expression of MMP-7 protein had significantly positive correlation with the expression of CD44v6 protein (r=0.568,P<0.05),the expression of MMP-7 protein had no correlation with the expression of nm23 protein (P>0.05), and the expression of nm23 protein had no correlation with the expression of CD44v6 protein (P>0.05). CONCLUSION:Despite their different mechanisms of action,heparanase,CD44v6 and nm23 may play important roles in the invasive infiltration and lymph node metastasis in gastric carcinomas.Instead of metastatic spreading,MMP- 7 may be just related to the invasion of gastric carcinomas. However,co-detection of these factors may be important to predict metastatic spreading in such patients.
基金
Supported by the National Natural Science Foundation of China,No 30271276
