摘要
从浙江洞头产羊栖菜中提取得到的褐藻糖胶,经DEAESepharoseCL 6B柱层析得F1、F2和F33个组分,F3又经SepharoseCL 6B柱层析得F31、F32和F333个级分.经凝胶过滤色谱检测,F31、F32和F33为均一组分.化学组成分析表明,这5个级分均为岩藻糖、半乳糖和甘露糖等糖基组成的杂多糖,并含有硫酸酯、糖醛酸以及少量的蛋白质,相对分子质量范围为2.5×104～9.5×105.F33中氮的质量分数为2%,氨基酸分析和紫外扫描表明氮大部分可能来自核酸.F32的硫酸酯对碱基本稳定,表明大部分硫酸酯位于单糖残基的垂直位置.
Fucoidan, separated from Sargassum fusiform (grown in Dongtou, Zhejiang Province), was graded into three fractions: F1, F2 and F3 by chromatography on a DEAE Sepharose CL-6B column. F3 was further graded into three fractions: F31, F32 and F33 by chromatography on a Sepharose CL-6B column, which were shown to be homogeneous by gel filtration chromatography. The five fractions all contained fucose, mannose and galactose predominantly, and also contained sulphate groups, uronic acid and little protein. Their Mw ranged from 25000 to 950000. F33 contained 2% N, most of which probably came from nucleic acid based on amino acid analysis and UV detectation. Ester sulphate in F32 was stable to alkali, which suggested most of sulphates were on the axial position of the sugars.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2004年第2期86-89,93,共5页
Journal of Wuxi University of Light Industry
