摘要
采用0.1mol/L的氢氧化钾对沙蒿籽胶多糖进行组分分级得到水溶多糖(AG1)和水不溶多糖(AG2)。利用离子交换柱层析从AG1中分离得到酸性多糖(AG11)和中性多糖(AG12)。经凝胶过滤色谱检测,AG11和AG12为均一组分。化学组成分析表明,AG1为葡萄糖糖基组成的葡聚糖,并含有糖醛酸;AG11和AG12分别为阿拉伯糖、鼠李糖、木糖、甘露糖、葡萄糖和半乳糖等糖基组成的杂多糖,并含有糖醛酸,相对分子质量分别为8.63×105Da和3.46×105Da。红外光谱测定表明,AG11、AG12和AG2具有多糖的特征吸收,并且其糖环均为吡喃环。
The polysaccharides ofArtemisia sphaerocephala Krasch gum were graded into water-soluble polysacchafide （AG1） and water-insoluble polysaccharide （AG2） with 0.1mol/L NaOH AGI 1 and AG12 were obtained with ion-exchange method from AG1, shown to be homogeneous by gel filtration chromatography. AG2 contains glucose predominantly, and also uronic acid. AG11 and AG12 contains arabinose, rhamnose, xylose, mannose, glucose and galactose predominantly and respectively, and also uronic acid. The molecular weights of AG11 and AG12 are 8.63 × 10^5Da and 3.46 × 10^5Da respectively. The structure of AG11, AG12 and AG2 contains pyranride and has the characteristic of glycoconjugate.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第3期73-76,共4页
Food Science
关键词
沙蒿籽胶
分级
纯化
结构
Artemisia sphaerocephala Krasch gum
fractionation
purification
structure
