摘要
目的 调查与SARS相关的卫生医疗机构内鼠形动物携带SARS冠状病毒 (CoV )情况 ,为寻找SARS的源头和传染途径提供证据。方法 常规方法捕捉老鼠 ,采用巢式PCR技术、荧光定量PCR技术、基因序列检定技术、病毒细胞培养技术进行测试。结果 在广州市与SARS相关的卫生医疗机构内捕捉的鼠形动物共 3种 5 9只 ;用SARS CoV特异性引物对 3 2份肛拭子样本进行PCR检测 ,结果显示肛拭子样本有 4份阳性 ,阳性率为 12 .5 % ;阳性片段基因序列与SARS CoV同源性为 90 %～ 96% ;PCR扩增片段阳性样本细胞培养结果未能分离到SARS CoV。结论 鼠形动物能否携带和传播SARS CoV ,仍需作进一步研究。
Objective To investigate SARS virus carried by murine-like animals in the hygienic units to provide evidence for looking for the origin and infectious ways of SARS virus. Methods Rats were caught in usual ways. Nested RT-PCR,Fluorescent quantitative PCR,Sequence analysis and virus isolation were applied. Results There are 59 murine-like animals of three species caught in the hygienic units related to SARS in Guangzhou. 32 samples of anus swabs test were tested in nested RT-PCR. The resnlts showed that four samples were positive. The positive rate was 12.5 percent. The sequence of positive parts had 90%～96% homogeneity with SARS-CoV. The result of the cell cultivation on positive samples of PCR amplification parts was negative. Conclusion It would be studied further to conform whether murine-like animals can carry and transmit SARS coronavirus.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2004年第1期7-9,共3页
Chinese Journal of Vector Biology and Control
基金
广东省SARS科技攻关项目(编号 :2003FD0 2-09)
