摘要
目的 调查广东重点地区鼠类和蜚蠊是否携带SARS冠状病毒或者是否SARS冠状病毒的宿主 ,为寻找SARS冠状病毒的来源及控制SARS流行提供依据。方法 巢式RT -PCR技术、荧光定量RT -PCR技术。结果 利用SARS冠状病毒特异性引物对广州、中山、江门及恩平等地的 160份鼠肺样品及 15份蜚蠊的体表擦拭子检测结果表明 ,荧光定量RT -PCR未检测到阳性 ,巢式RT -PCR显示来自广州市的 1份蜚蠊体表擦拭子呈可疑阳性。结论 巢式RT -PCR、荧光定量RT -PCR都适合于病媒生物SARS冠状病毒的初步筛选 ,目前未有明显证据显示鼠类及蜚蠊携带和传播SARS冠状病毒 ,仍需作进一步研究。
Objective To investigate SARS coronavirus carried by or reserved in rat and cockroach, and try to provide the evidence for the origin and control of SARS coronavirus. Methods Nested reverse-transcript PCR and fluorescent quantitative reverse-transcript PCR were applied. Results With SARS Cov.-specific primer, a total of 160 rat lung tissues and 15 cockroach surface swabs were tested in nested RT-PCR and fluorescent quantitative RT-PCR. No positive was found by fluorescent quantitative RT-PCR, and only one uncertain positive result of cockroach surface by nested RT-PCR. Conclusion Nested RT-PCR and fluorescent quantitative RT-PCR are available in fast testing positive vector, and there was no evidence to regard the role of rat and cockroach in the origin and transmission of SARS coronavirus, which deserves to be studied further in the future.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2003年第5期332-334,共3页
Chinese Journal of Vector Biology and Control
基金
广东省SARS科技攻关项目 (编号 :2 0 0 3FD0 2 - 0 9)
