摘要
本文主要应用单细胞培养技术培育紫菜新品系,以建立紫菜细胞工程快速育苗系统。通过酶解技术分离出紫菜叶状体细胞,并进行多克隆,从中获得4个细胞纯系植株(HA,HB,HC,HD)。对该4个细胞株系进行了纯系培养,并对其细胞苗和丝状体的生长速度和抗高温(19℃,21℃,23℃,25℃)性进行了测定。结果显示,HB株系具有较高的抗高温性和最快生长速度。在1998年到2000年间,在江苏省启东县海丰海区对HB株系进行了海上栽培实验,结果显示,实验组的产量高于当地对照组产量,证明了条斑紫菜HB株系不仅具有较高的抗高温性,而且也有较快生长速度。本实验说明应用细胞工程进行条斑紫菜育种是一条很好的快速育种途径。
A new breeding technology with cell culture in Porphyra yezoensis was studied, to establish a system of fast breeding with cell engineering in Porphyra. By means of somatic cell isolation and multiple clone technique, 4 pure cell-lines (HA, HB, HC, HD) have been established in Porphyra yezoensis. With purely culturing the cell seedlings and conchocelis filaments of the cell lines, their growth rate and resistance to higher temperature were measured. Among cell line HA, HB, HC, HD, HB was the best for resisting higher temperature (at 19℃,21℃,23℃,25℃). Also the growth rate of HB was faster than others. In 1998 - 2000, the HB was cultivated at sea field of Haifeng in Qi-dong County, Jiangsu Province. The yield of HB was higher than that of local cultivar. So the HB might be a good cell line for both resisting higher temperature and faster growth. It showed the breeding with cell culture was a fast breeding method.
出处
《实验生物学报》
CSCD
北大核心
2003年第3期191-196,共6页
Acta Biologiae Experimentalis Sinica
基金
Supported by International Foundation for Science (IFS), Sweden and by Agriculture Minister of China
