摘要
目的观察热休克预处理对严重烧伤大鼠胃黏膜的影响。方法热休克预处理诱导热休克蛋白产生,然后制作烧伤大鼠急性胃黏膜损伤模型,进行胃黏膜损伤指数评分。运用免疫组化方法,检测胃黏膜热休克蛋白的表达,并进行图像分析。结果热休克预处理组胃黏膜损伤积分显著低于对照组(P<0.01)。免疫组化分析显示其胃黏膜热休克蛋白表达较对照组明显增强(P<0.01)。结论热休克预处理对烧伤大鼠胃黏膜具有保护作用,其保护机制可能与热休克蛋白诱导表达增强有关。
Objective To investigate the effects of heat shock pretreatment on the gastric mucosa in severely burned rats. Methods After heat shock proteins (HSPs) were induced by heat shock pretreatment, burn-induced gastric mucosal lesion model was established according to the protocol of Kitajima. Gastric mu-cosal lesion index (LI) was measured in light microscopy. The expression of HSPs was analyzed with immuno-histochemical method. Results Gastric mucosal lesion indexes in heat shock pretreatment group were significantly lower than that in burn group (P < 0.01). The expression of HSPs of heat shock pretreatment group was higher than that of burn group (P<0.01). Conclusion Heat shock pretreatment is of protective role to acute gastric mucosal lesions in burned rats and the mechanism might be closely related to the enhanced expression of induced HSPs.
出处
《现代消化及介入诊疗》
2004年第1期11-13,共3页
Modern Interventional Diagnosis and Treatment in Gastroenterology
