摘要
采用ELISA的方法检测35例泛发性皮肤瘙痒症患者的外周血单核细胞(PBMC)在体外经PHA诱导后T辅助细胞不同功能亚群细胞因子IL-4、IL-6及IFN-γ的分泌,同时以20例正常人的PBMC作对照.结果表明:泛发性皮肤瘙痒症患者PBMC中T辅助-2型(Th2)的细胞因子IL-4的产生明显高于正常对照(P<0.05),而T辅助-1型(Th1)的细胞因子IFN-γ的产生明显低于正常对照(P<0.05),细胞因子IL-6的产生在泛发性皮肤瘙痒症患者PBMC与正常对照的PBMC中无明显差异(P>0.05).同时,患者的瘙痒程度评分分数与其IL-4的产生呈正相关,而与其γ-IFN的产生呈负相关(P值均<0.05).
The production of interleukin-4, interleukin-6 and interferon-γ in peripheral blood mononuclear cells of generalized pruritus patients and normal controls after PHA stimulation in vitro were measured by ELISA methods. The results showed the production of Th2 cytokine (interleukin-4 )were significantly increased in PBMC of generalized pruritus patients compared with normal controls(P<0.05). In contrast, the production of Th1 cytokine (interferon-γ) in patients with generalized pruritus was significantly lower than in normal controls(P<0.05), whereas no significant difference in interleukin-6 production was found between patients with generalized pruritus and normal controls (P>0.05). The pruritus score scale in patients was positively correlated with its PBMC's interleukin-4 production, but negatively correlated with interferon-γ prodution (P<0.05).
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2004年第2期202-204,共3页
Journal of Zhejiang University:Agriculture and Life Sciences
