摘要
目的 :观察弥漫性脑损伤后海马齿状回新生神经元GAD67和Fos蛋白的表达 ,探讨海马齿状回新生神经元的功能特点。方法 :参考Marmarou方法制作大鼠弥漫性脑损伤模型 ,采用BrdU标记和免疫荧光组织化学方法并结合激光共聚焦显微镜技术观察海马齿状回中BrdU阳性细胞GAD67的表达和二次致伤后Fos蛋白的表达情况。结果 :①成年大鼠弥漫性脑损伤后海马齿状回颗粒细胞层中的BrdU免疫阳性细胞可表达GAD67,而位于亚颗粒增生带的BrdU免疫阳性细胞未见GAD67表达 ;②在病理刺激下 ,成年大鼠弥漫性脑损伤后齿状回颗粒细胞层内的BrdU免疫阳性细胞可表达Fos蛋白。结论 :弥漫性脑损伤后齿状回颗粒细胞层中的新生神经元不仅可以表达神经活性递质而且能够被病理刺激激活 ,具有与成熟神经元相似的功能特点。
Objective: To explore the function characteristics of new-born neurons in the dentate gyrus induced by diffuse brain injury (DBI). Methods: Male mature SpragueDawley rats were subjected to DBI model. Bromodeoxyuridine (BrdU) labelling method and Fluorescence immunohistochemical method combined with laser confocal microscopy were used to observe the Fos protein expression induced by secondary insults and GAD67 expression of BrdU-positive cells in the dentate gyrus (DG). Results: ①The result of confocal microscopy showed that most of BrdU-positive cells induced by DBI were distributed in the granular cell layer, and expressed GAD67. A few BrdU-positive cells in the subgranular proliferative zone could not express GAD67. ②Under pathological stress, most of BrdU-positive cells induced by DBI in the dentate gyrus granular cell layer could express Fos protein. Conclusion: The new-born neurons in the dentate gyrus induced by DBI can not only express neural active transmitter, but also be activated by pathological stress. It suggest that the new-born neurons in the dentate gyrus induced by DBI have the parallel function characteristics with around neurons.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2004年第2期203-205,209,共4页
Chinese Journal of Clinical Anatomy
基金
中国人民武装警察部队科研立项项目 (WKH2 0 0 30 30 )
关键词
弥漫性脑损伤
神经发生
齿状回
谷氨酸脱羧酶
FOS蛋白
diffuse brain injury
neurogenesis
dentate gyrus
glutamic acid decarboxylase
fos protein
