摘要
作者利用A PAGE(Acid polyacrylamidegelelectrophoresis)法研究了来自中国西藏的野生二棱大麦、野生瓶形大麦和野生六棱大麦共106份材料在3个醇溶蛋白位点的遗传多样性.结果表明,所有供试材料在Ⅰ、Ⅱ和Ⅲ组醇溶蛋白位点分别发现了26、14和22种等位变异类型,平均等位变异数大小顺序为:野生二棱大麦,野生瓶形大麦,野生六棱大麦.这些等位变异类型的频率在这3组野生大麦间的分布存在显著或极显著差异.野生二棱大麦平均遗传多样性程度极显著地高于野生六棱大麦,这3组大麦间存在明显的遗传分化.最后,讨论了栽培大麦的起源中心及中国栽培大麦的起源与进化问题.本研究结果表明,野生二棱大麦是栽培大麦的祖先,支持了栽培大麦起源的"二棱大麦单系发生论".
The author studied genetic diversity in the storage protein hordein in seeds from 106 accessions of wild barley in Tibet, including 50 accessions of the two-rowed wild barley,Hordeum vulgaressp. spontaneum(HS), 29 accessions of the six-rowed wild barley H. agriocrithon(HA) and 27 accessions of the bottle-shaped wild barley H. lagunculiforme(HL), using A-PAGE (Acid-polyacrylamide gel electrophoresis) method. The results showed that, altogether 26,14 and 22 different phenotypes were found at Ⅰ, Ⅱ and Ⅲ hordein groups, respectively. The order for both the mean of number of alleles and the amount of genetic diversity was HS>HL>HA, the frequencies distributions of these phenotypes among the three groups of wild berley showed significant or highly significant differences. The mean degree of genetic diversity was very significantly higher in HS than in HA, and genetic differentiation was significantly existed among the three barley groups. Finally, the issues for the centers of origin of cultivated barley and the origin and evolution of Chinese cultivated barley were discussed by a comparison of our results with those of previous studies, definitely supporting the hypothesis of that two-rowed wild barley is primary progenitor of cultivated barley-the theory of two-rowed barley monophyletic origin.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2004年第2期440-445,共6页
Journal of Sichuan University(Natural Science Edition)
基金
四川省科技厅和四川省教育厅经费资助
关键词
西藏
大麦
醇溶蛋白
遗传多样性
进化
Tibet
Hordeum vulgareL.
hordein
genetic diversity
evolution
